BRENDA - Enzyme Database show
show all sequences of 1.13.11.3

Intergeneric evolutionary homology revealed by the study of protocatechuate 3,4-dioxygenase from Azotobacter vinelandii

Durham, D.R.; Stirling, L.A.; Ornsten, L.N.; Perry, J.J.; Biochemistry 19, 149-155 (1980)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,3-Dihydroxybenzoate
-
Azotobacter vinelandii
3,4-dihydroxyphenylacetic acid
-
Azotobacter vinelandii
4-(dimethylamino)benzaldehyde
-
Azotobacter vinelandii
4-hydroxybenzoate
-
Azotobacter vinelandii
4-methylcatechol
-
Azotobacter vinelandii
4-nitrocatechol
-
Azotobacter vinelandii
caffeic acid
-
Azotobacter vinelandii
catechol
-
Azotobacter vinelandii
Protocatechualdehyde
-
Azotobacter vinelandii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
protocatechuate
-
Azotobacter vinelandii
0.0275
-
O2
-
Azotobacter vinelandii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
10 g-atoms per mol enzyme
Azotobacter vinelandii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23300
-
alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE
Azotobacter vinelandii
25500
-
alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE
Azotobacter vinelandii
480000
-
ultracentrifugation
Azotobacter vinelandii
510000
-
gel filtration
Azotobacter vinelandii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Azotobacter vinelandii
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, DEAE-cellulose, Bio-gel agarose, DEAE-cellulose
Azotobacter vinelandii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
46.69
-
-
Azotobacter vinelandii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dihydroxybenzoate + O2
-
439501
Azotobacter vinelandii
3-carboxy-cis,cis-muconate
-
439501
Azotobacter vinelandii
?
3-methylcatechol + O2
at 5% the rate of protocatechuic acid oxidation
439501
Azotobacter vinelandii
2-methylmuconate
-
439501
Azotobacter vinelandii
?
catechol + O2
at 3% the rate of protocatechuic acid oxidation
439501
Azotobacter vinelandii
muconate
-
439501
Azotobacter vinelandii
?
pyrogallol + O2
at 10% the rate of protocatechuic acid oxidation
439501
Azotobacter vinelandii
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
decamer
alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE
Azotobacter vinelandii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 8.5, 90 min, stable
Azotobacter vinelandii
60
-
pH 8.5, 10 min, 90% loss of activity
Azotobacter vinelandii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Azotobacter vinelandii
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8.5
-
50°C, 90 min stable
Azotobacter vinelandii
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0006
-
4-nitrocatechol
-
Azotobacter vinelandii
0.001
-
Protocatechualdehyde
-
Azotobacter vinelandii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,3-Dihydroxybenzoate
-
Azotobacter vinelandii
3,4-dihydroxyphenylacetic acid
-
Azotobacter vinelandii
4-(dimethylamino)benzaldehyde
-
Azotobacter vinelandii
4-hydroxybenzoate
-
Azotobacter vinelandii
4-methylcatechol
-
Azotobacter vinelandii
4-nitrocatechol
-
Azotobacter vinelandii
caffeic acid
-
Azotobacter vinelandii
catechol
-
Azotobacter vinelandii
Protocatechualdehyde
-
Azotobacter vinelandii
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0006
-
4-nitrocatechol
-
Azotobacter vinelandii
0.001
-
Protocatechualdehyde
-
Azotobacter vinelandii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
protocatechuate
-
Azotobacter vinelandii
0.0275
-
O2
-
Azotobacter vinelandii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
10 g-atoms per mol enzyme
Azotobacter vinelandii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23300
-
alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE
Azotobacter vinelandii
25500
-
alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE
Azotobacter vinelandii
480000
-
ultracentrifugation
Azotobacter vinelandii
510000
-
gel filtration
Azotobacter vinelandii
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, DEAE-cellulose, Bio-gel agarose, DEAE-cellulose
Azotobacter vinelandii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
46.69
-
-
Azotobacter vinelandii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dihydroxybenzoate + O2
-
439501
Azotobacter vinelandii
3-carboxy-cis,cis-muconate
-
439501
Azotobacter vinelandii
?
3-methylcatechol + O2
at 5% the rate of protocatechuic acid oxidation
439501
Azotobacter vinelandii
2-methylmuconate
-
439501
Azotobacter vinelandii
?
catechol + O2
at 3% the rate of protocatechuic acid oxidation
439501
Azotobacter vinelandii
muconate
-
439501
Azotobacter vinelandii
?
pyrogallol + O2
at 10% the rate of protocatechuic acid oxidation
439501
Azotobacter vinelandii
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
decamer
alpha,beta, 5 * 23300 + 5 * 25500, SDS-PAGE
Azotobacter vinelandii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
pH 8.5, 90 min, stable
Azotobacter vinelandii
60
-
pH 8.5, 10 min, 90% loss of activity
Azotobacter vinelandii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Azotobacter vinelandii
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8.5
-
50°C, 90 min stable
Azotobacter vinelandii
Other publictions for EC 1.13.11.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741660
Zhang
Improvement of the Stabilizat ...
Rhizobium sp. LMB-1
Appl. Biochem. Biotechnol.
183
1035-1048
2017
-
-
1
-
-
1
9
2
-
1
1
1
-
2
-
-
1
-
-
-
-
-
1
1
1
1
1
2
1
1
-
-
-
-
-
-
-
1
-
-
-
1
-
9
-
2
-
1
1
1
-
-
-
1
-
-
-
-
1
1
1
1
1
2
1
1
-
-
-
-
-
-
-
-
742982
Mulla
-
Biodegradation of 3-chloroben ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) OS13
J. Environ. Chem. Eng.
4
1423-1431
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
743818
Das
Highly efficient and stable n ...
Pseudomonas putida
Sci. Rep.
6
33572
2016
-
-
-
-
-
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
4
1
-
1
-
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
4
1
-
1
-
1
2
1
-
-
-
-
-
-
-
2
2
742063
Sainsbury
Chemical intervention in bact ...
Rhodococcus jostii
Bioorg. Chem.
60
102-109
2015
-
-
-
-
-
-
6
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
3
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
742046
Guzik
Degradation potential of prot ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia KB2
BioMed Res. Int.
2014
138768
2014
4
-
-
-
1
-
16
-
-
5
-
2
-
5
-
-
-
-
-
-
-
1
15
-
3
1
-
-
2
3
-
-
-
-
-
4
-
-
-
-
1
-
-
16
-
-
-
5
-
2
-
-
-
-
-
-
-
1
15
-
3
1
-
-
2
3
-
-
1
1
1
1
-
-
743122
Guzik
Protocatechuate 3,4-dioxygena ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia KB2
J. Mol. Microbiol. Biotechnol.
24
150-160
2014
4
-
-
-
-
-
4
1
-
-
-
2
-
5
-
-
-
-
-
-
-
-
15
1
1
-
-
-
1
1
-
-
-
-
-
4
-
-
-
-
-
-
-
4
-
1
-
-
-
2
-
-
-
-
-
-
-
-
15
1
1
-
-
-
1
1
-
-
1
-
-
1
-
-
725655
Sim
-
Purification and characterizat ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes KF707
J. Korean Soc. Appl. Biol. Chem.
56
401-408
2013
-
-
-
-
-
-
-
10
-
-
3
4
-
20
-
-
1
-
-
-
1
-
16
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
3
4
-
-
-
1
-
-
1
-
16
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
726481
Da Silva
-
The effects of trace elements, ...
Leifsonia sp.
Sci. Agric. (Piracicaba, Braz.)
70
68-73
2013
-
-
1
-
1
-
-
-
-
2
-
1
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
1
2
-
-
-
-
-
-
-
-
726535
Guzik
Influence of metal ions on bio ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia KB2
World J. Microbiol. Biotechnol.
29
267-273
2013
-
-
-
-
-
-
8
-
-
1
-
4
-
10
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
712295
Zhao
PcaO positively regulates pcaH ...
Corynebacterium glutamicum
J. Bacteriol.
192
1565-1572
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
702976
Guzik
Isolation and characterization ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia KB2
Braz. J. Microbiol.
40
285-291
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704290
Zimmermann
Role of Acinetobacter baylyi C ...
Acinetobacter baylyi, Acinetobacter baylyi Crc
J. Bacteriol.
191
2834-2842
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
686803
Kim
Molecular cloning and function ...
Chromohalobacter sp. HS-2, Chromohalobacter sp. HS2
FEMS Microbiol. Lett.
280
235-241
2008
-
-
-
-
-
-
-
-
-
-
-
4
-
7
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697244
Bubinas
-
Degradation of naphthalene by ...
Geobacillus sp.
Cent. Eur. J. Biol.
3
61-68
2008
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685485
Bubinas
-
Protocatechuate 3,4-dioxygenas ...
Geobacillus sp.
Biologija (Vilnius)
53
31-34
2007
-
1
-
-
-
-
-
2
-
-
1
-
-
1
-
-
1
-
-
1
1
-
3
-
1
1
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
1
-
3
-
1
1
1
-
1
1
-
-
-
-
-
-
-
-
687286
Pau
Spectroscopic and electronic s ...
Brevibacterium fuscum, Pseudomonas putida
J. Am. Chem. Soc.
129
1944-1958
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671470
Gore
Deletion mutations caused by D ...
Acinetobacter baylyi, Acinetobacter baylyi ADP1
Appl. Environ. Microbiol.
72
5239-5245
2006
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674004
Kurahashi
Trigonal-bipyramidal geometry ...
Pseudomonas putida
Inorg. Chem.
45
7709-7721
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674180
Borowski
Mechanism for Catechol Ring Cl ...
Pseudomonas putida
J. Am. Chem. Soc.
128
12941-12953
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671672
Parulekar
-
Multiple pathways for the biod ...
Pseudomonas mendocina
Asian J. Microbiol. Biotechnol. Environ. Sci.
7
85-89
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671966
Valley
Roles of the equatorial tyrosy ...
Pseudomonas putida
Biochemistry
44
11024-11039
2005
-
-
1
-
4
-
2
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
5
1
-
-
-
1
-
-
-
-
1
-
-
4
-
-
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
677115
Shen
Key enzymes of the protocatech ...
Corynebacterium glutamicum
Sci. China C Life Sci.
48
241-249
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657551
Brown
Biophysical analyses of design ...
Acinetobacter sp., Pseudomonas putida
Annu. Rev. Microbiol.
58
555-585
2004
-
-
-
2
7
-
-
7
-
1
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
2
7
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
695732
Buchan
Diverse organization of genes ...
Roseovarius nubinhibens ISM, Ruegeria pomeroyi, Sagittula stellata E-37, Sulfitobacter sp.
Appl. Environ. Microbiol.
70
1658-1668
2004
-
-
1
-
-
-
-
-
-
-
-
4
-
7
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439514
Kahng
Enhanced detection and charact ...
Acinetobacter lwoffii, Acinetobacter lwoffii K24
Biochem. Biophys. Res. Commun.
295
903-909
2002
-
-
-
-
-
-
-
-
-
-
2
-
-
6
-
-
1
-
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439513
Contzen
Cloning of the genes for a 4-s ...
Agrobacterium tumefaciens, Hydrogenophaga palleronii
Mol. Microbiol.
41
199-205
2001
-
-
2
-
3
-
-
-
-
-
2
-
-
8
-
-
-
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439510
Buchan
Key aromatic-ring-cleaving enz ...
Roseobacter sp., Sagittula stellata
Appl. Environ. Microbiol.
66
4662-4672
2000
-
-
1
-
-
-
-
-
-
-
2
-
-
7
-
-
-
-
-
-
5
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
5
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439511
Patil
The use of protocatechuate dio ...
Burkholderia cepacia
Anal. Biochem.
286
187-192
2000
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439512
Vetting
Structure of Acinetobacter str ...
Acinetobacter sp.
Biochemistry
39
7943-7955
2000
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439509
D'Argenio
Substitution, insertion, delet ...
Acinetobacter sp.
J. Bacteriol.
181
6478-6487
1999
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439508
Frazee
The axial tyrosinate Fe3+ liga ...
no activity in Pseudomonas fluorescens, Pseudomonas putida
Biochemistry
37
2131-2144
1998
-
-
1
1
1
-
-
6
-
-
-
-
-
3
-
-
1
-
-
-
1
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
439506
Orville
Crystal structures of substrat ...
Pseudomonas putida
Biochemistry
36
10052-10066
1997
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439507
Orville
Cyanide and nitric oxide bindi ...
Pseudomonas putida
Biochemistry
36
14044-14055
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439505
Hammer
Purification and characterizat ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens S2, Hydrogenophaga palleronii, Hydrogenophaga palleronii S1
Arch. Microbiol.
166
92-100
1996
-
-
-
-
-
-
4
5
-
-
-
-
-
10
-
-
2
-
-
-
3
-
26
-
-
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
5
-
-
-
-
-
-
-
2
-
-
3
-
26
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
666009
Ohlendorf
Structure of protocatechuate 3 ...
Pseudomonas aeruginosa
J. Mol. Biol.
244
586-608
1994
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439484
Whittaker
Protocatechuate 3,4-dioxygenas ...
Brevibacterium fuscum
Methods Enzymol.
188
82-88
1990
1
-
-
-
-
-
-
2
-
1
3
1
-
2
-
-
1
-
-
-
1
1
2
1
-
-
-
2
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
1
3
1
-
-
-
1
-
-
1
1
2
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
439497
Orville
Binding of isotopically labele ...
Brevibacterium fuscum
J. Biol. Chem.
264
8791-8801
1989
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439498
Wojtas-Wasilewska
-
Immobilization of protocatechu ...
Pleurotus ostreatus
Phytochemistry
27
2731-2733
1988
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
2
-
1
-
2
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
2
1
-
-
2
-
-
-
-
-
-
-
-
-
439493
Ohlendorf
Determination of the quaternar ...
Pseudomonas putida
J. Mol. Biol.
195
225-227
1987
-
-
-
1
-
-
-
-
-
1
3
-
-
2
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439492
Ludwig
Characterization of crystals o ...
Burkholderia cepacia
J. Biol. Chem.
259
14840-14842
1984
-
-
-
1
-
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439499
Chen
-
Aromatic metabolism in Rhizobi ...
Rhizobium leguminosarum
Arch. Microbiol.
138
187-190
1984
-
-
-
-
-
-
3
1
-
1
3
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
1
3
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439502
Kurane
-
Protocatechuate 3,4-dioxygenas ...
Nocardia erythropolis
Agric. Biol. Chem.
48
2105-2111
1984
-
-
-
-
-
-
6
1
-
-
1
-
-
1
-
-
1
-
-
-
1
-
7
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
-
-
1
-
-
-
-
1
-
-
1
-
7
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-
439503
Whittaker
Brevibacterium fuscum protocat ...
Brevibacterium fuscum
J. Biol. Chem.
259
4466-4475
1984
-
-
-
1
-
-
-
2
-
1
3
-
-
2
-
1
1
-
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
1
3
-
-
-
1
1
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
439494
May
-
Protocatechuate 3,4-dioxygenas ...
Pseudomonas sp.
Biochemistry
22
5331-5340
1983
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439496
Walsh
Halogenated protocatechuates a ...
Burkholderia cepacia
J. Biol. Chem.
258
14413-14421
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439490
May
Protocatechuate 3,4-dioxygenas ...
Pseudomonas putida
J. Biol. Chem.
257
12746-12751
1982
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439491
Iwaki
The primary structure of the b ...
Pseudomonas aeruginosa
Arch. Biochem. Biophys.
210
210-223
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439504
Bull
Purification and properties of ...
Pseudomonas putida
J. Biol. Chem.
256
12673-12680
1981
-
-
-
-
-
-
4
5
-
1
5
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
1
5
-
-
-
-
-
-
-
-
-
1
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
439501
Durham
Intergeneric evolutionary homo ...
Azotobacter vinelandii
Biochemistry
19
149-155
1980
-
-
-
-
-
-
9
2
-
1
4
-
-
8
-
-
1
-
-
-
1
-
4
1
-
-
2
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
-
9
2
2
-
1
4
-
-
-
-
1
-
-
1
-
4
1
-
-
2
-
1
-
1
-
-
-
-
-
-
-
665455
Satyshur
Preliminary crystallographic s ...
Pseudomonas aeruginosa
J. Biol. Chem.
255
10015-10016
1980
-
-
-
1
-
-
-
-
-
1
2
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439488
Mohan
-
Purification and properties of ...
Tecoma stans
Plant Sci. Lett.
16
267-272
1979
-
-
-
-
-
-
3
2
1
1
1
-
-
1
-
-
1
-
-
1
1
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
1
1
1
-
-
-
-
1
-
1
1
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
439489
Kohlmiller
The primary structure of the a ...
Pseudomonas putida
J. Biol. Chem.
254
7302-7308
1979
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439495
May
Protocatechuate 3,4-dioxygenas ...
Pseudomonas aeruginosa
Biochemistry
18
5933-5939
1979
-
-
-
-
-
-
6
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
6
6
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439487
May
Interaction of protocatechuate ...
Pseudomonas putida
Biochemistry
17
1853-1860
1978
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439486
Que
Protocatechuate 3,4-dioxygenas ...
Pseudomonas putida
Biochim. Biophys. Acta
485
60-74
1977
-
-
-
-
-
-
14
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
-
-
14
13
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439500
Hou
Protocatechuate 3, 4-dioxygena ...
Acinetobacter calcoaceticus, Acinetobacter calcoaceticus 80-1
Biochemistry
15
582-588
1976
-
-
-
-
-
-
19
2
-
1
1
-
-
5
-
-
1
-
-
1
1
1
2
-
1
-
1
-
1
-
1
-
7
-
-
-
-
-
-
-
-
-
-
19
7
2
-
1
1
-
-
-
-
1
-
1
1
1
2
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
439485
Zaborsky
Immobilization of protocatechu ...
Pseudomonas aeruginosa
Biochim. Biophys. Acta
289
68-76
1972
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
439480
Gibson
-
Assay of enzymes of aromatic m ...
Pseudomonas sp.
Methods Enzymol.
6A
463-478
1971
-
-
-
1
-
1
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439483
Fujisawa
Protocatechuate 3,4-dioxygenas ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa B-10
J. Biol. Chem.
243
2673-2681
1968
-
-
-
1
-
-
4
2
-
1
1
-
-
2
-
-
1
-
-
-
1
-
11
-
-
-
1
1
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
4
1
2
-
1
1
-
-
-
-
1
-
-
1
-
11
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
439481
Stanier
Protocatechuic acid oxidase ...
Pseudomonas fluorescens, Pseudomonas fluorescens A.3.12.
J. Biol. Chem.
210
799-808
1954
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
1
-
-
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
1
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
439482
Gross
The metabolism of protocatechu ...
Neurospora crassa
J. Biol. Chem.
219
781-796
1954
-
-
-
-
-
2
3
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-