Literature summary for 1.13.11.20 extracted from

Davies, C.G.; Fellner, M.; Tchesnokov, E.P.; Wilbanks, S.M.; Jameson, G.N.
The Cys-Tyr cross-link of cysteine dioxygenase changes the optimal pH of the reaction without a structural change (2014), Biochemistry, 53, 7961-7968 .

Search for more literature for 1.13.11.20

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of wild-type and mutant Strep-tagged enzyme	Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hanging drop vapour diffusion method, mixing of 0.0015 ml of 7 mg/mL CDO mutant C93G in 10 mM sodium phosphate and 20 mM NaCl, pH 7.5, and 0.0006 ml of crushed wild-type CDO crystal seeds in their own growth solution consisting of 25% w/v PEG 1500, 13 mM succinate, 44 mM sodium phosphate, and 44 mM glycine, pH 5.2, with 0.0015 ml of reservoir buffer containing 26% w/v PEG 4000, 200 mM ammonium acetate, and 100 mM sodium citrate, pH 6.3, equilibration against reservoir solution, X-ray diffraction structure determination and analysis at 1.82 A resolution	Rattus norvegicus

Crystallization (Comment)

Organism

purified enzyme, hanging drop vapour diffusion method, mixing of 0.0015 ml of 7 mg/mL CDO mutant C93G in 10 mM sodium phosphate and 20 mM NaCl, pH 7.5, and 0.0006 ml of crushed wild-type CDO crystal seeds in their own growth solution consisting of 25% w/v PEG 1500, 13 mM succinate, 44 mM sodium phosphate, and 44 mM glycine, pH 5.2, with 0.0015 ml of reservoir buffer containing 26% w/v PEG 4000, 200 mM ammonium acetate, and 100 mM sodium citrate, pH 6.3, equilibration against reservoir solution, X-ray diffraction structure determination and analysis at 1.82 A resolution

Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
C93G	site-directed mutagenesis, structure comparison to the wild-type enzyme	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	steady-state Michaelis-Menten kinetics	Rattus norvegicus
0.002	-	L-cysteine	20% cross-linked wild-type enzyme, pH 9.1, temperature not specified in the publication	Rattus norvegicus
0.003	-	L-cysteine	not cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication	Rattus norvegicus
0.005	-	L-cysteine	60% cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	active site bound, reconstitution of the purified enzyme	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-cysteine + O2	Rattus norvegicus	-	3-sulfinoalanine	-	?

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	P21816	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant Strep-tagged wild-type and mutant enzyme by affinity chromatography to over 95% purity	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-cysteine + O2	-	Rattus norvegicus	3-sulfinoalanine	-	?

Synonyms

Synonyms	Comment	Organism
CDO	-	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.12	-	L-cysteine	20% cross-linked wild-type enzyme, pH 9.1, temperature not specified in the publication	Rattus norvegicus
0.25	-	L-cysteine	60% cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication	Rattus norvegicus
0.33	-	L-cysteine	not cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	pH-dependence of wild-type and mutant enzyme kinetics, detailed overview	Rattus norvegicus
7.7	-	60% cross-linked wild-type enzyme	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
Cys-Tyr cofactor	-	Rattus norvegicus

General Information

General Information	Comment	Organism
additional information	modeling the pH-dependence of cysteine dioxygenation	Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.056	-	L-cysteine	20% cross-linked wild-type enzyme, pH 9.1, temperature not specified in the publication	Rattus norvegicus
0.056	-	L-cysteine	60% cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication	Rattus norvegicus
0.095	-	L-cysteine	not cross-linked wild-type enzyme, pH 7.7, temperature not specified in the publication	Rattus norvegicus