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Literature summary for 1.13.11.2 extracted from

  • Hupert-Kocurek, K.; Wojcieszynska, D.; Guzik, U.
    Altering substrate specificity of catechol 2,3-dioxygenase from Planococcus sp. strain S5 by random mutagenesis (2014), Acta Biochim. Pol., 61, 705-710 .
    View publication on PubMed
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Planococcus sp. S5

Protein Variants

Protein Variants Comment Organism
A23T/F212S the mutant shows 40.65% activity with catechol compared to the wild type enzyme Planococcus sp. S5
F191I/C268R/Y272H/V280A/Y293D the mutant shows 38.29% activity with catechol compared to the wild type enzyme Planococcus sp. S5
H24R/F168S/Q275R the mutant shows 19.66% activity with catechol compared to the wild type enzyme Planococcus sp. S5
R296Q the mutant shows 128.47% activity with catechol compared to the wild type enzyme. The mutation also improves the activity of the enzyme against 4-chlorocatechol (582.04% activity compared to the wild type enzyme) Planococcus sp. S5

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Planococcus sp. S5
-
 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Planococcus sp. S5 E7DDG2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00073
-
 mutant enzyme A23T/F212S, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.0009
-
 mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00108
-
 mutant enzyme H24R/F168S/Q275R, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00138
-
 mutant enzyme R296Q, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.0018
-
 wild type enzyme, with 3-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00272
-
 mutant enzyme A23T/F212S, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00298
-
 mutant enzyme H24R/F168S/Q275R, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00547
-
 mutant enzyme H24R/F168S/Q275R, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00572
-
 mutant enzyme H24R/F168S/Q275R, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00806
-
 mutant enzyme A23T/F212S, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00811
-
 mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00827
-
 mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00878
-
 mutant enzyme A23T/F212S, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.00963
-
 wild type enzyme, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.01487
-
 mutant enzyme F191I/C268R/Y272H/V280A/Y293D, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.01966
-
 wild type enzyme, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.02062
-
 wild type enzyme, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.02223
-
 mutant enzyme R296Q, with 4-methylcatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.02775
-
 mutant enzyme R296Q, with catechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5
0.05605
-
 mutant enzyme R296Q, with 4-chlorocatechol as substrate, at pH 7.5, temperature not specified in the publication Planococcus sp. S5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2
-
 Planococcus sp. S5 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
4-chlorocatechol + O2
-
 Planococcus sp. S5 4-chloro-2-hydroxymuconate semialdehyde
-
 ?
4-methylcatechol + O2
-
 Planococcus sp. S5 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
catechol + O2
-
 Planococcus sp. S5 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

Synonyms Comment Organism
C23o
-
 Planococcus sp. S5