Protein Variants | Comment | Organism |
---|---|---|
E266Q | specific activity is less than 0.1% of that of the wild-type enzyme, binds 33% of the wild-type level of manganese | Arthrobacter globiformis |
H155A | specific activity is less than 0.1% of that of the wild-type enzyme, binds 0.4% of the wild-type level of manganese | Arthrobacter globiformis |
H214A | specific activity is less than 0.1% of that of the wild-type enzyme, binds 1.8% of the wild-type level of manganese | Arthrobacter globiformis |
H42A | 97% of wild-type activity at 18°C, 30% of wild-type activity at 37°C | Arthrobacter globiformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Manganese | enzyme is dependent on manganese, the conserved residues H155, H1214 and E266 ligate manganese | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Arthrobacter globiformis CM-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Arthrobacter globiformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
- |
Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxyphenylacetate + O2 | - |
Arthrobacter globiformis | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Arthrobacter globiformis CM-2 | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? |