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Literature summary for 1.12.99.6 extracted from
- Critical aspects of [NiFe]hydrogenase ligand composition (2009), Dalton Trans., 22, 4304-4309.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the active site has a characteristic bis(micro-thiolato)NiFe unit, where the Ni atom and the Fe atom are bridged by an undetermined oxygen-bearing ligand. This ligand probably derives from the aqueous solvent and is therefore most likely to be H2O, OH- or O2-. A NiFe complex is not able to activate H2 when coordinated with an CH3CN ligand, thus a highly labile ligand that is simultaneously able to act as a Lewis base for the heterolytic activation of H2 is crucial to the action of H2ase. The CH3CN-coordinated Ni(II)Fe(II) complex is unstable in the presence of water and decomposed to the Ni(II) complex and the Fe(II) complex via the Fe-S bond cleavage in water. In order to synthesise H2O-coordinated NiFe complexes in aqueous media, the Lewis acidity of the Fe centre must be increased to form strong Fe-S bonds. Thus, organometallic ligands with a back-donating character to form Fe-C bonds are required | Megalodesulfovibrio gigas |  |
| Ni2+ | the active site has a characteristic bis(micro-thiolato)NiFe unit, where the Ni atom and the Fe atom are bridged by an undetermined oxygen-bearing ligand. This ligand probably derives from the aqueous solvent and is therefore most likely to be H2O, OH- or O2-. A NiFe or NiRu complex are not able to activate H2 when coordinated with an CH3CN ligand, thus a highly labile ligand that is simultaneously able to act as a Lewis base for the heterolytic activation of H2 is crucial to the action of H2ase. The CH3CN-coordinated Ni(II)Fe(II) complex is unstable in the presence of water and decomposed to the Ni(II) complex and the Fe(II) complex via the Fe-S bond cleavage in water. In order to synthesise H2O-coordinated NiFe complexes in aqueous media, the Lewis acidity of the Fe centre must be increased to form strong Fe-S bonds. Thus, organometallic ligands with a back-donating character to form Fe-C bonds are required | Megalodesulfovibrio gigas | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Megalodesulfovibrio gigas | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| [NiFe]H2ase | - |
Megalodesulfovibrio gigas |
| [NiFe]hydrogenase | - |
Megalodesulfovibrio gigas |
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