Cloned (Comment) | Organism |
---|---|
mutant enzymes expressed in Escherichia coli | Cupriavidus necator |
Protein Variants | Comment | Organism |
---|---|---|
F110L | 18% of wild-type H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation | Cupriavidus necator |
I62V | 6% of wild-type H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation | Cupriavidus necator |
I62V/F110L | mutant enzyme shows no H2 uptake activity. The loss of activity of the mutant protein originates from reversible oxidative inactivation | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen tolerance is based on limited access of oxygen to the active site | Cupriavidus necator |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + acceptor | - |
Cupriavidus necator | H+ + reduced acceptor | - |
? | |
H2 + acceptor | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | H+ + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
H2-sensing [NiFe] hydrogenase | - |
Cupriavidus necator |