KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
H2 | - |
Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Cupriavidus necator | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 4Fe-4S cluster | Cupriavidus necator | |
Ni | NiFe-hydrogenase | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE | Cupriavidus necator |
55000 | - |
alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE | Cupriavidus necator |
165000 | - |
gel filtration | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Oxidation Stability | Organism |
---|---|
enzyme is extremely sensitive to inactivation by O2 | Cupriavidus necator |
Purification (Comment) | Organism |
---|---|
purified protein forms a complex with histidine protein kinase | Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.942 | - |
- |
Cupriavidus necator |
Storage Stability | Organism |
---|---|
4°C, 20% loss of activity after 48 h under argon | Cupriavidus necator |
4°C, 50% loss of activity after 12 h under an atmosphere of 100% H2 | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + acceptor | benzyl viologen acts as electron acceptor | Cupriavidus necator | H+ + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2,beta2, 2 * 37000 + 2 * 55000, SDS-PAGE | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
H2 production | Cupriavidus necator |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | H2 uptake is constant over a pH range from pH 5 to pH 10 | Cupriavidus necator |