BRENDA - Enzyme Database
show all sequences of 1.12.98.4

Purification and properties of an extremely thermostable membrane-bound sulfur-reducing complex from the hyperthermophilic Pyrodictium abyssi

Dirmeier, R.; Keller, M.; Frey, G.; Huber, H.; Stetter, K.O.; Eur. J. Biochem. 252, 486-491 (1998)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no quinione, but enzyme complex contains cytochrome c and has two additional cytochromes, probably of b-type
Pyrodictium abyssi
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-(n-heptyl)-4-hydroxyquinoline N-oxide
not affected by
Pyrodictium abyssi
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pyrodictium abyssi
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu
1.2 mol per mol protein
Pyrodictium abyssi
Fe
50-55 mol iron per mol protein
Pyrodictium abyssi
Ni
1.6 mol per mol protein
Pyrodictium abyssi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
enzym complex, gel-filtration
Pyrodictium abyssi
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrodictium abyssi
-
TAG 11, a sulfur-reducing complex
-
Oxidation Stability
Oxidation Stability
Organism
the sulfur-reducing activity is irreversibly damaged by oxygen, anaerobic conditions: no loss of activity after one week, aerobic conditions: 80% loss of activity in 24 h
Pyrodictium abyssi
Purification (Commentary)
Commentary
Organism
enzyme complex
Pyrodictium abyssi
Reaction
Reaction
Commentary
Organism
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1
a sulfur-reducing complex including hydrogenase, a sulfur reductase and electron-transferring components described
Pyrodictium abyssi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.56
-
at 100°C
Pyrodictium abyssi
Storage Stability
Storage Stability
Organism
room temperature, exposed to air, 24 h, 80% loss of activity
Pyrodictium abyssi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfur + electron donor
electron donor: molecular hydrogen
437778
Pyrodictium abyssi
H2S + oxidized electron donor
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
nine major polypeptides with molecular masses of 82000, 72000, 65000, 50000, 47000, 42000, 40000, 30000 and 24000 Da
Pyrodictium abyssi
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
below 85°C about 10% loss of activity; enzyme extremely thermostable
Pyrodictium abyssi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Pyrodictium abyssi
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.5
-
Pyrodictium abyssi
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no quinione, but enzyme complex contains cytochrome c and has two additional cytochromes, probably of b-type
Pyrodictium abyssi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-(n-heptyl)-4-hydroxyquinoline N-oxide
not affected by
Pyrodictium abyssi
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pyrodictium abyssi
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu
1.2 mol per mol protein
Pyrodictium abyssi
Fe
50-55 mol iron per mol protein
Pyrodictium abyssi
Ni
1.6 mol per mol protein
Pyrodictium abyssi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
enzym complex, gel-filtration
Pyrodictium abyssi
Oxidation Stability (protein specific)
Oxidation Stability
Organism
the sulfur-reducing activity is irreversibly damaged by oxygen, anaerobic conditions: no loss of activity after one week, aerobic conditions: 80% loss of activity in 24 h
Pyrodictium abyssi
Purification (Commentary) (protein specific)
Commentary
Organism
enzyme complex
Pyrodictium abyssi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.56
-
at 100°C
Pyrodictium abyssi
Storage Stability (protein specific)
Storage Stability
Organism
room temperature, exposed to air, 24 h, 80% loss of activity
Pyrodictium abyssi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfur + electron donor
electron donor: molecular hydrogen
437778
Pyrodictium abyssi
H2S + oxidized electron donor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
nine major polypeptides with molecular masses of 82000, 72000, 65000, 50000, 47000, 42000, 40000, 30000 and 24000 Da
Pyrodictium abyssi
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
below 85°C about 10% loss of activity; enzyme extremely thermostable
Pyrodictium abyssi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Pyrodictium abyssi
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
9.5
-
Pyrodictium abyssi
Other publictions for EC 1.12.98.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716255
Yang
SurR regulates hydrogen produc ...
Pyrococcus furiosus
Mol. Microbiol.
77
1111-1122
2010
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1
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1
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674514
Guiral
A membrane-bound multienzyme, ...
Aquifex aeolicus
J. Biol. Chem.
280
42004-42015
2005
1
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4
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659878
Laska
Membrane-bound hydrogenase and ...
Acidianus ambivalens
Microbiology
149
2357-2371
2003
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1
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437780
Keller
Hydrogen-sulfur oxidoreductase ...
Pyrodictium abyssi
Methods Enzymol.
331
442-451
2001
1
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4
1
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1
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1
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1
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1
1
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1
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437781
Laska
Improved purification of the m ...
Acidianus ambivalens
J. Chromatogr. B
737
151-160
2000
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437782
Ng
Purification and some properti ...
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans NASF-1
J. Biosci. Bioeng.
90
199-203
2000
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6
1
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439641
Ma
Characterization of hydrogenas ...
Pyrococcus furiosus
J. Bacteriol.
182
1864-1871
2000
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1
2
5
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4
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1
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1
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437773
Klimmek
The single cysteine residue of ...
Wolinella succinogenes
Eur. J. Biochem.
263
79-84
1999
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4
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745391
Silva
On the prosthetic groups of t ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
4
284-291
1999
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745603
Selvaggi
-
In vitro hydrogen photoproduc ...
Pyrococcus furiosus
J. Photochem. Photobiol. A
125
107-112
1999
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1
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437778
Dirmeier
Purification and properties of ...
Pyrodictium abyssi
Eur. J. Biochem.
252
486-491
1998
1
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1
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3
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1
1
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1
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437779
Sugio
Purification and characterizat ...
thermophilic iron-oxidizing bacterium strain TI-1
Biosci. Biotechnol. Biochem.
62
705-709
1998
2
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7
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1
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675504
Schneider
Crystal structure of dimethyl ...
Rhodobacter capsulatus
J. Mol. Biol.
263
53-69
1996
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437776
Arendsen
Redox properties of the sulfhy ...
Pyrococcus furiosus
FEBS Lett.
368
117-121
1995
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723118
Pedroni
Characterization of the locus ...
Pyrococcus furiosus
Microbiology
141
449-458
1995
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437774
Fauque
-
Sulfur reductases from spirill ...
Sulfurospirillum deleyianum, Wolinella succinogenes
Methods Enzymol.
243
367-383
1994
3
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437775
Jankielewicz
-
Polysulfide reductase and form ...
Wolinella succinogenes
Arch. Microbiol.
162
238-242
1994
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437777
Childers
Characterization and regulatio ...
Thermotoga neapolitana
Appl. Environ. Microbiol.
60
2622-2626
1994
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722289
Ma
-
Hydrogen production from pyruv ...
Pyrococcus furiosus
FEMS Microbiol. Lett.
122
245-250
1994
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4
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439664
Ma
Hydrogenase of the hyperthermo ...
Pyrococcus furiosus
Proc. Natl. Acad. Sci. USA
90
5341-5344
1993
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437772
Zöphel
Investigations on microbial su ...
Sulfurospirillum deleyianum
Eur. J. Biochem.
195
849-856
1991
2
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2
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2
1
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1
2
1
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1
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-
-
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4
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-
-
-
-
-
-
-
-
-
-
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2
-
-
-
-
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2
-
-
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2
1
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1
2
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1
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4
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437770
Zöphel
-
Investigations on microbial su ...
Desulfomicrobium baculatum, Desulfomicrobium baculatum 9974, Desulfuromonas acetexigens, Desulfuromonas acetexigens 2873, Desulfuromonas acetoxidans, Desulfuromonas acetoxidans 5071, Desulfuromusa succinoxidans, Desulfuromusa succinoxidans Go20, Sulfurospirillum deleyianum
Arch. Microbiol.
150
72-77
1988
4
-
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7
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10
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1
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10
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1
1
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5
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16
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1
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4
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7
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10
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1
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1
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5
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16
-
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1
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437771
Schröder
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Isolation of the sulphur reduc ...
Wolinella succinogenes
Arch. Microbiol.
149
572-579
1988
1
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3
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2
1
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1
1
1
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1
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1
1
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1
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3
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2
1
2
1
1
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1
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1
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1
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1
1
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