Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain FB810 | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
C169S | decreased activity | Schizosaccharomyces pombe |
C48S | completely inactive | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | high concentrations of H2O2 temporarily inactivate Tpx1 | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | O74887 | - |
- |
Oxidation Stability | Organism |
---|---|
inactivation of Tpx1 by oxidation of its catalytic cysteine to a sulfinic acid is always preceded by a sulfinic acid form in a covalently linked dimer | Schizosaccharomyces pombe |
Purification (Comment) | Organism |
---|---|
GSH-Sepharose 4B bead chromatography | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 + reduced dithiothreitol | - |
Schizosaccharomyces pombe | H2O + oxidized dithiothreitol | - |
? | |
H2O2 + reduced thioredoxin | - |
Schizosaccharomyces pombe | H2O + oxidized thioredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
2-Cys peroxiredoxin | - |
Schizosaccharomyces pombe |
TPx1 | - |
Schizosaccharomyces pombe |
two-cysteine peroxiredoxin | - |
Schizosaccharomyces pombe |