Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Vibrio cholerae |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M potassium thiocyanate and 20% (w/v) PEG 3000 | Vibrio cholerae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
guaiacol | at pH 8.0 and 25°C | Vibrio cholerae | |
0.05 | - |
Reactive Blue 19 | at pH 8.0 and 25°C | Vibrio cholerae | |
0.18 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 8.0 and 25°C | Vibrio cholerae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Vibrio cholerae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae | Q9KQ59 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Vibrio cholerae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Vibrio cholerae | ? | - |
? | |
guaiacol + H2O2 + H+ | - |
Vibrio cholerae | ? | - |
? | |
Reactive Blue 19 + H2O2 + H+ | - |
Vibrio cholerae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | Vibrio cholerae |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Vibrio cholerae |
DyP | - |
Vibrio cholerae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
guaiacol | at pH 8.0 and 25°C | Vibrio cholerae | |
1.3 | - |
Reactive Blue 19 | at pH 8.0 and 25°C | Vibrio cholerae | |
500 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 8.0 and 25°C | Vibrio cholerae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Vibrio cholerae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
Reactive Blue 19 | at pH 8.0 and 25°C | Vibrio cholerae |