Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium carbonate | 50 mM, 125% of initial activity | Streptomyces griseosporeus | |
tetraacetylethylenediamine | 25 mM, 121% of initial activity | Streptomyces griseosporeus | |
Triton X-100 | 5% w/v, 123% of initial activity | Streptomyces griseosporeus |
Application | Comment | Organism |
---|---|---|
degradation | enzyme shows marked dye-decolorization efficiency and stability toward denaturing, oxidizing, and bleaching agents, and compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C | Streptomyces griseosporeus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | 1% w/v, 72% residual activity | Streptomyces griseosporeus | |
sodium perborate | 5% w/v, 71% residual activity | Streptomyces griseosporeus | |
Urea | 1% w/v, 85% residual activity | Streptomyces griseosporeus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
2,4-Dichlorophenol | pH 8.5, 60°C | Streptomyces griseosporeus | |
0.46 | - |
o-Dianisidine | pH 8.5, 60°C | Streptomyces griseosporeus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces griseosporeus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseosporeus | - |
- |
- |
Streptomyces griseosporeus SN9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification from culture supernatant | Streptomyces griseosporeus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.75 | - |
substrate 2,4-dichlorophenol, pH 8.5, temperature not specified in the publication | Streptomyces griseosporeus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 guaiacol + H2O2 | 27% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
2 guaiacol + H2O2 | 27% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus SN9 | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | 92% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | ? + H2O | - |
? | |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | 92% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus SN9 | ? + H2O | - |
? | |
2,4,6-trichlorophenol + H2O2 | 63% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | ? + H2O | - |
? | |
2,4,6-trichlorophenol + H2O2 | 63% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus SN9 | ? + H2O | - |
? | |
2,4-dichlorophenol + H2O2 | best substrate | Streptomyces griseosporeus | ? + H2O | - |
? | |
4-chlorophenol + H2O2 | 45% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | ? + H2O | - |
? | |
humic acid + H2O2 | - |
Streptomyces griseosporeus | ? + H2O | - |
? | |
humic acid + H2O2 | - |
Streptomyces griseosporeus SN9 | ? + H2O | - |
? | |
o-dianisidine + H2O2 | 76% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus | ? + H2O | - |
? | |
o-dianisidine + H2O2 | 76% of the activity with 2,4-dichlorophenol | Streptomyces griseosporeus SN9 | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Streptomyces griseosporeus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces griseosporeus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
2,4-Dichlorophenol | pH 8.5, 60°C | Streptomyces griseosporeus | |
1.16 | - |
o-Dianisidine | pH 8.5, 60°C | Streptomyces griseosporeus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptomyces griseosporeus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | spectrum shows a heme peak at 409 nm | Streptomyces griseosporeus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.57 | - |
2,4-Dichlorophenol | pH 8.5, 60°C | Streptomyces griseosporeus | |
2.53 | - |
o-Dianisidine | pH 8.5, 60°C | Streptomyces griseosporeus |