Cloned (Comment) | Organism |
---|---|
the triple deletion mutant gpx1delta/gpx2delta/gpx3delta is not growing with medium containing unsaturated linolenate | Saccharomyces cerevisiae |
wild-type enzyme gpx1, gpx2, gpx3 and mutants, expression in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutants of gpx1delta, gpx2delta and gpx3delta showing diminished enzyme activity, triple mutant gpx1delta/gpx2delta/gpx3delta has almost no remaining activity | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
alpha-tocopherol stabilizes triple mutant against H2O2 and unsaturated linolenate | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + lipid hydroperoxide | Saccharomyces cerevisiae | protection of biomembranes against oxidative damage | glutathione disulfide + lipid + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
genes GPX1, GPX2 and GPX3 | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutant proteins | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + lipid hydroperoxide | - |
Saccharomyces cerevisiae | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + lipid hydroperoxide | protection of biomembranes against oxidative damage | Saccharomyces cerevisiae | glutathione disulfide + lipid + H2O | - |
? |