Application | Comment | Organism |
---|---|---|
industry | the enzyme decoloures up to six different industrial dyes, with or without the use of redox mediators such as ABTS | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0153 | - |
syringaldazine | 40°C, pH 6.0 | Thermus thermophilus | |
0.167 | - |
syringaldazine | 35°C, pH 6.0 | Thermus thermophilus | |
0.256 | - |
syringaldazine | 30°C, pH 6.0 | Thermus thermophilus | |
2.4 | - |
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | 60°C, pH 6.0 | Thermus thermophilus | |
3.8 | - |
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | 55°C, pH 6.0 | Thermus thermophilus | |
4.4 | - |
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | 50°C, pH 6.0 | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
SDS-PAGE | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72HW2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2 | - |
Thermus thermophilus | ? | - |
? | |
4-fluoro-2-methylphenol + O2 | - |
Thermus thermophilus | ? | - |
? | |
additional information | the purified enzyme is able to decolourize Green Dye, Orange Dye and Acid Red Dye. The enzyme is unable to decolourize NBB, RBB, or Congo Red. However, with 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) (5 mM) acting as redox mediator, the enzyme decolourizes NBB, RBB, or Congo Red | Thermus thermophilus | ? | - |
? | |
syringaldazine + O2 | - |
Thermus thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 5300, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
TthLAC | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
reaction time: 180 min | Thermus thermophilus |
90 | - |
reactions time: less than 1 h | Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 85 | 40°C: about 40% of maximal activity, 85°C: about 55% of maximal activity | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme undergoes slow partial unfolding and thermal inactivation above 65°C, making it unsuitable for long incubations above this temperature | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.58 | - |
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | 50°C, pH 6.0 | Thermus thermophilus | |
3.33 | - |
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | 55°C, pH 6.0 | Thermus thermophilus | |
3.66 | - |
syringaldazine | 30°C, pH 6.0 | Thermus thermophilus | |
4.77 | - |
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | 60°C, pH 6.0 | Thermus thermophilus | |
5.33 | - |
syringaldazine | 35°C, pH 6.0 | Thermus thermophilus | |
10.3 | - |
syringaldazine | 40°C, pH 6.0 | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Thermus thermophilus |