Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | 20% (v/v), 15% increase inactivity | Trichoderma harzianum | |
Tween-40 | 0.5% (v/v), 10% increase in activity | Trichoderma harzianum |
Application | Comment | Organism |
---|---|---|
degradation | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment | Trichoderma harzianum |
environmental protection | laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents. The wide pH- and thermostability attributes of immobilized laccase make them suitable for environmental applications | Trichoderma harzianum |
General Stability | Organism |
---|---|
Ca-alginate, Cu-alginate, Ca-alginate-chitosan and solĀgel immobilized laccase show an operational stability of 36%, 51%, 66% and 82%, respectively after 6 cycles | Trichoderma harzianum |
soluble enzyme loses 45% of its activity after 8 days at 4Ā°C. Sol-gel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively | Trichoderma harzianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, 15% loss of activity | Trichoderma harzianum | |
acetone | 20% (v/v), 42% loss of activity | Trichoderma harzianum | |
Al3+ | 5 mM, 60% loss of activity | Trichoderma harzianum | |
Cd2+ | 5 mM, 78% loss of activity | Trichoderma harzianum | |
Co2+ | 5 mM, 67% loss of activity | Trichoderma harzianum | |
dithiothreitol | 5 mM, 65% loss of activity | Trichoderma harzianum | |
EDTA | 5 mM, 70% loss of activity | Trichoderma harzianum | |
ethanol | 20% (v/v), 15% loss of activity | Trichoderma harzianum | |
Fe2+ | 5 mM, 60% loss of activity | Trichoderma harzianum | |
Hg2+ | 5 mM, 90% loss of activity | Trichoderma harzianum | |
Isopropanol | 20% (v/v), 30% loss of activity | Trichoderma harzianum | |
L-cysteine | 5 mM, 20% loss of activity | Trichoderma harzianum | |
methanol | 20% (v/v), 20% loss of activity | Trichoderma harzianum | |
additional information | soluble laccase shows more than 80% relative activity in organic solvents like ethanol, methanol, toluene and acetonitrile | Trichoderma harzianum | |
Ni2+ | 5 mM, 35% loss of activity | Trichoderma harzianum | |
SDS | 0.5%, 60% loss of activity | Trichoderma harzianum | |
Toluene | 20% (v/v), 18% loss of activity | Trichoderma harzianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6, 25Ā°C, soluble enzyme | Trichoderma harzianum | |
2 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6, 25Ā°C,laccase immobilized in solĀgel matrix | Trichoderma harzianum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trichoderma harzianum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 18% increase in activity | Trichoderma harzianum | |
Cu2+ | 5 mM, 85% increase in activity | Trichoderma harzianum | |
Mg2+ | 5 mM, 15% increase in activity | Trichoderma harzianum | |
Mn2+ | 5 mM, 8% increase in activity | Trichoderma harzianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | - |
- |
- |
Trichoderma harzianum HZN10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Trichoderma harzianum |
Specific Activity Minimum [Āµmol/min/mg] | Specific Activity Maximum [Āµmol/min/mg] | Comment | Organism |
---|---|---|---|
164 | - |
pH 6, 25Ā°C | Trichoderma harzianum |
Storage Stability | Organism |
---|---|
4Ā°C, 8 days, soluble enzyme loses 45% of its activity. SolĀgel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively | Trichoderma harzianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trichoderma harzianum | ? | - |
? | |
2,6-dimethoxyphenol + O2 | 96% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
catechol + O2 | 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
catechol + O2 | 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum HZN10 | ? | - |
? | |
ferulic acid + O2 | 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
ferulic acid + O2 | 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum HZN10 | ? | - |
? | |
gallic acid + O2 | 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
gallic acid + O2 | 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum HZN10 | ? | - |
? | |
guaiacol + O2 | 83% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
additional information | free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator | Trichoderma harzianum | ? | - |
? | |
additional information | free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator | Trichoderma harzianum HZN10 | ? | - |
? | |
syringaldazine + O2 | 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
syringaldazine + O2 | 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum HZN10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Trichoderma harzianum |
Temperature Optimum [Ā°C] | Temperature Optimum Maximum [Ā°C] | Comment | Organism |
---|---|---|---|
50 | - |
soluble and immobilized laccases | Trichoderma harzianum |
Temperature Minimum [Ā°C] | Temperature Maximum [Ā°C] | Comment | Organism |
---|---|---|---|
50 | 70 | all the forms of immobilized laccase show more than 90% relative activity at higher temperature of 55Ā°C and 60Ā°C. Sol-gel immobilized laccase shows 97% (65Ā°C) and 94% (70Ā°C) relative activity at higher temperatures. Soluble enzyme loses maximum activity above 55Ā°C | Trichoderma harzianum |
Temperature Stability Minimum [Ā°C] | Temperature Stability Maximum [Ā°C] | Comment | Organism |
---|---|---|---|
50 | - |
4 h, soluble and immobilized laccases retain more than 90% activity at 50Ā°C. Copper alginate shows better stability than calcium alginate immobilized laccase | Trichoderma harzianum |
55 | - |
4 h, calcium alginate-chitosan immobilized laccase retains 91% activity | Trichoderma harzianum |
65 | - |
4 h, sol-gel immobilized laccase retains 90% activity | Trichoderma harzianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | laccase immobilized in sol-gel matrix | Trichoderma harzianum |
5 | 6 | copper alginate and calcium alginate-chitosan immobilized laccase | Trichoderma harzianum |
6 | - |
soluble enzyme and calcium alginate immobilized enzyme | Trichoderma harzianum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
4 h, solĀgel immobilized laccase retains 98% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀgel matrix > calcium alginateĀchitosan > copper alginate > calcium alginate | Trichoderma harzianum |
5 | 6 | 4 h, solĀgel immobilized laccase retains 100% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀgel matrix > calcium alginateĀchitosan > copper alginate > calcium alginate | Trichoderma harzianum |
7 | - |
4 h, solĀgel immobilized laccase retains 96% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀgel matrix > calcium alginateĀchitosan > copper alginate > calcium alginate | Trichoderma harzianum |
8 | - |
4 h, solĀgel immobilized laccase retains 92% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀgel matrix > calcium alginateĀchitosan > copper alginate > calcium alginate | Trichoderma harzianum |