Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Heptyl-4-hydroxyquinoline-N-oxide | - |
Gluconobacter oxydans | |
antimycin A | 56% residual activity at 0.005 mg/ml; 56% residual activity at 0.005 mg/ml | Gluconobacter oxydans | |
aurachin C 1-10 | 15% residual activity at 0.005 mg/ml; 15% residual activity at 0.005 mg/ml | Gluconobacter oxydans | |
aurachin D | 16% residual activity at 0.005 mg/ml; 16% residual activity at 0.005 mg/ml | Gluconobacter oxydans | |
cyanide | quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration; quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration | Gluconobacter oxydans | |
Gramicidin S | 67% residual activity at 0.005 mg/ml; 67% residual activity at 0.005 mg/ml | Gluconobacter oxydans | |
LL-Z1272gamma | 69% residual activity at 0.005 mg/ml; 69% residual activity at 0.005 mg/ml | Gluconobacter oxydans | |
additional information | not inhibited by ascofuranone; not inhibited by ascofuranone | Gluconobacter oxydans | |
Piericidin A | 14% residual activity at 0.005 mg/ml; 14% residual activity at 0.005 mg/ml | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0403 | - |
ubiquinol-1 | at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Gluconobacter oxydans | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37813 | - |
1 * 53583 + 1 * 37813, calculated from amino acid sequence | Gluconobacter oxydans |
44000 | - |
1 * 54000 + 1 * 44000, SDS-PAGE | Gluconobacter oxydans |
53583 | - |
1 * 53583 + 1 * 37813, calculated from amino acid sequence | Gluconobacter oxydans |
54000 | - |
1 * 54000 + 1 * 44000, SDS-PAGE | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
subunit I; formerly Gluconobacter suboxydans strain IFO12528 | - |
Gluconobacter oxydans | Q5FU84 | subunit II; formerly Gluconobacter suboxydans strain IFO12528. The enzyme from strain 621H is expressed in strain NBRC3172 | - |
Gluconobacter oxydans 621H | Q5FU84 | subunit II; formerly Gluconobacter suboxydans strain IFO12528. The enzyme from strain 621H is expressed in strain NBRC3172 | - |
Gluconobacter oxydans NBRC 3172 | - |
subunit I; formerly Gluconobacter suboxydans strain IFO12528 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquinol-1 + O2 | - |
Gluconobacter oxydans | ubiquinone-1 + H2O | - |
? | |
ubiquinol-1 + O2 | - |
Gluconobacter oxydans 621H | ubiquinone-1 + H2O | - |
? | |
ubiquinol-1 + O2 | - |
Gluconobacter oxydans NBRC 3172 | ubiquinone-1 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 53583 + 1 * 37813, calculated from amino acid sequence | Gluconobacter oxydans |
heterodimer | 1 * 54000 + 1 * 44000, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
CIO | - |
Gluconobacter oxydans |
CioAB | - |
Gluconobacter oxydans |
cyanide-insensitive oxidase | - |
Gluconobacter oxydans |
cyanide-insensitive quinol oxidase | - |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme b558, heme b595 and heme d | Gluconobacter oxydans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00004 | - |
at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans | aurachin C 1-10 | |
0.0007 | - |
at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans | Piericidin A | |
0.008 | - |
quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration with the IC50 of 0.008 mM (relative amplitude, 21%) and 0.013 mM (79%), at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans | cyanide | |
0.013 | - |
at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans | 2-Heptyl-4-hydroxyquinoline-N-oxide | |
0.013 | - |
at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans | LL-Z1272gamma | |
0.017 | - |
at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans | antimycin A | |
0.04 | - |
at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans | Gramicidin S | |
13 | - |
quinol oxidase activity of the membranes from the wild type shows a biphasic dependence on the cyanide concentration with the IC50 of 0.008 mM (relative amplitude, 21%) and 13 mM (79%), at 25°C in 50 mM potassium phosphate (pH 6.5) | Gluconobacter oxydans | cyanide |