Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | 0.066 mM, 26%, 59% and 96% inhibition of isoenzymes B, C, and D, respectively | Prunus persica | |
CN- | 0.165 mM, 84%, 70%, 100% and 40% inhibition of isoenzymes A, B, C and D, respectively | Prunus persica | |
diethyldithiocarbamate | 0.066 mM, 31%, 40%, 36% and 84% inhibition of isoenzymes A, B, C, and D, respectively | Prunus persica | |
EDTA | 100 mM, 33%, 60%, 81% and 35% inhibition of isoenzymes A, B, C and D, respectively | Prunus persica | |
glutathione | 0.066 mM, 2%, 22% and 84% inhibition of isoenzymes B, C, and D, respectively | Prunus persica | |
NaCl | 800 mM, 48%, 47%, 55% and 93% inhibition of isoenzymes A, B, C, and D, respectively | Prunus persica | |
NaHSO3 | 0.066 mM, 64%, 50% and 27% inhibition of isoenzymes B, C, and D, respectively | Prunus persica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
catechol | isoenzyme B | Prunus persica | |
6.6 | - |
catechol | isoenzyme A | Prunus persica | |
7 | - |
catechol | isoenzyme C | Prunus persica | |
36 | - |
catechol | isoenzyme D | Prunus persica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus persica | - |
peach | - |
Purification (Comment) | Organism |
---|---|
isoenzymes A, B, C and D, acetone precipitate, DEAE-cellulose | Prunus persica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Prunus persica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isoenzyme A, 0.0016 units, isoenzyme B, 0.011 units, isoenzyme C, 0.0013 units/mg, isoenzyme D, 0.0028 units, 1 unit is defined as absorbance change at 420 nm of 0.001 per min | Prunus persica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4,5-trihydroxybenzoic acid + O2 | isoenzymes A-C | Prunus persica | ? | - |
? | |
3,4,5-trihydroxybenzoic acid + O2 | trivial name gallic acid | Prunus persica | ? | - |
? | |
4-methylcatechol + O2 | isoenzymes A-D | Prunus persica | 4-methyl-o-benzoquinone | - |
? | |
caffeic acid + 1/2 O2 | isoenzymes C and D | Prunus persica | caffeoyl quinone + H2O | - |
? | |
catechol + 1/2 O2 | isoenzymes A, B, C and D show no phenolase activity | Prunus persica | 1,2-benzoquinone + H2O | - |
? | |
chlorogenic acid + O2 | isoenzymes A-D | Prunus persica | ? | - |
? | |
D-catechin + O2 | isoenzymes A-D | Prunus persica | ? | - |
? | |
dopamine + O2 | isoenzymes A-D | Prunus persica | 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O | - |
? | |
L-dopa + 1/2 O2 | isoenzymes A-D | Prunus persica | L-dopaquinone + H2O | - |
? | |
protocatechuic acid + O2 | isoenzyme C | Prunus persica | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-lives of isoenzymes A, B and D: 5.4, 14,6 and 14.1 min respectively, isoenzyme C: stable for 50 min at 55°C, half-life at 76°C: 2.2 min | Prunus persica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
isoenzyme B | Prunus persica |
6.8 | - |
isoenzyme A | Prunus persica |
7 | - |
isoenzyme D | Prunus persica |
7.2 | - |
isoenzyme C | Prunus persica |