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Literature summary for 1.1.99.B3 extracted from

  • Foumani, M.; Vuong, T.V.; MacCormick, B.; Master, E.R.
    Enhanced polysaccharide binding and activity on linear beta-glucans through addition of carbohydrate-binding modules to either terminus of a glucooligosaccharide oxidase (2015), PLoS ONE, 10, e0125398.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.99.B3

Cloned(Commentary)

Cloned (Comment) Organism
expressed as fusion protein with carbohydrate binding modules CBM3, CBM11, and CBM44 from Clostridium thermocellum in Pichia pastoris GS115 cells Sarocladium strictum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum

Organism

Organism UniProt Comment Textmining
Sarocladium strictum D7PF15
-
-
Sarocladium strictum CBS 346.70 D7PF15
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Sarocladium strictum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Avicel + O2
-
 Sarocladium strictum ?
-
 ?
Avicel + O2
-
 Sarocladium strictum CBS 346.70 ?
-
 ?
beta-(1->3,1->4)-glucan + O2
-
 Sarocladium strictum ?
-
 ?
beta-(1->3,1->4)-glucan + O2
-
 Sarocladium strictum CBS 346.70 ?
-
 ?
carboxymethyl cellulose + O2
-
 Sarocladium strictum ?
-
 ?
carboxymethyl cellulose + O2
-
 Sarocladium strictum CBS 346.70 ?
-
 ?
cellotetraose + O2
-
 Sarocladium strictum cellotetraono-1,5-lactone + H2O2
-
 ?
cellotetraose + O2
-
 Sarocladium strictum CBS 346.70 cellotetraono-1,5-lactone + H2O2
-
 ?
cellulose + O2
-
 Sarocladium strictum ?
-
 ?
cellulose + O2
-
 Sarocladium strictum CBS 346.70 ?
-
 ?
glucomannan + O2
-
 Sarocladium strictum ?
-
 ?
xyloglucan + O2
-
 Sarocladium strictum ?
-
 ?

Synonyms

Synonyms Comment Organism
GOOX
-
 Sarocladium strictum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.17
-
 cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
56.7
-
 cellotetraose wild type enzyme, at pH 8.0 and 37°C Sarocladium strictum