Protein Variants | Comment | Organism |
---|---|---|
R277G | kcat for (S)-mandelate is 178fold lower than wild-type value, KM-value for (S)-mandelate is 160fold higher than wild-type value, KM-value for {(S)-3-phenyllactate is 1.6fold lower than wild-type value | Pseudomonas putida |
R277H | kcat for (S)-mandelate is 1000fold lower than wild-type value, KM-value for (S)-mandelate is 608fold higher than wild-type value | Pseudomonas putida |
R277K | kcat for (S)-mandelate is 3.7fold lower than wild-type value, KM-value for (S)-mandelate is 36.7fold higher than wild-type value | Pseudomonas putida |
R277L | kcat for (S)-mandelate is 421fold lower fold than wild-type value, KM-value for (S)-mandelate is 392fold higher than wild-type value | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
(S)-3-phenyllactate | pH 7.5, 20°C, mutant enzyme R277G | Pseudomonas putida | |
0.12 | - |
(S)-Mandelate | pH 7.5, 20°C, wild-type enzyme | Pseudomonas putida | |
4.3 | - |
(S)-3-phenyllactate | pH 7.5, 20°C, wild-type enzyme | Pseudomonas putida | |
4.4 | - |
(S)-Mandelate | pH 7.5, 20°C, mutant enzyme R277K | Pseudomonas putida | |
19.2 | - |
(S)-Mandelate | pH 7.5, 20°C, mutant enzyme R277H | Pseudomonas putida | |
47 | - |
(S)-Mandelate | pH 7.5, 20°C, mutant enzyme R277G | Pseudomonas putida | |
73 | - |
(S)-Mandelate | pH 7.5, 20°C, mutant enzyme R277L | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzymes R277K, R277G, R277H and R277L | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-phenyllactate + acceptor | - |
Pseudomonas putida | ? | - |
? | |
(S)-mandelate + acceptor | - |
Pseudomonas putida | 2-oxo-2-phenylacetate + reduced acceptor | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
(S)-Mandelate | pH 7.5, 20°C, mutant enzyme R277L | Pseudomonas putida | |
0.64 | - |
(S)-Mandelate | pH 7.5, 20°C, mutant enzyme R277G | Pseudomonas putida | |
1.52 | - |
(S)-Mandelate | pH 7.5, 20°C, mutant enzyme R277H | Pseudomonas putida | |
73 | - |
(S)-Mandelate | pH 7.5, 20°C, mutant enzyme R277K | Pseudomonas putida | |
201 | - |
(S)-3-phenyllactate | pH 7.5, 20°C, mutant enzyme R277G | Pseudomonas putida | |
270 | - |
(S)-Mandelate | pH 7.5, 20°C, wild-type enzyme | Pseudomonas putida | |
270 | - |
(S)-3-phenyllactate | pH 7.5, 20°C, wild-type enzyme | Pseudomonas putida |