BRENDA - Enzyme Database show
show all sequences of 1.1.99.12

Isolation and Characterization of a New Quinoprotein Dehydrogenase, L-Sorbose/L-Sorbosone Dehydrogenase.

Asakura, A.; Hoshino, T.; Biosci. Biotechnol. Biochem. 63, 46-53 (1999)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CoCl2
strain DSM 4025, 5 mM, 84% inhibition
Gluconobacter oxydans
EDTA
strain DSM 4025, 5 mM, 87% inhibition
Gluconobacter oxydans
EGTA
strain DSM 4025, 5 mM, 89% inhibition
Gluconobacter oxydans
FeCl2
strain DSM 4025, 5 mM, 89% inhibition
Gluconobacter oxydans
FeCl3
strain DSM 4025, 5 mM, 97% inhibition
Gluconobacter oxydans
NiSO4
strain DSM 4025, 5 mM, 77% inhibition
Gluconobacter oxydans
ZnCl2
strain DSM 4025, 5 mM, 90% inhibition
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
1-propanol
strain DSM 4025
Gluconobacter oxydans
230
-
L-sorbose
strain DSM 4025
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62500
-
strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
Gluconobacter oxydans
64500
-
strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
Gluconobacter oxydans
135000
-
strain DSM 4025, gel filtration
Gluconobacter oxydans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-sorbose + acceptor
Gluconobacter oxydans
strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol
L-sorbosone + reduced acceptor
-
Gluconobacter oxydans
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
strain DSM 4025
-
Purification (Commentary)
Commentary
Organism
strain DSM 4025, PEG 600, DEAE-Toyopearl, Q-Sepharose
Gluconobacter oxydans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.73
-
strain DSM 4025
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + acceptor
strain DSM 4025, 69.4% of activity with 1-propanol
287728
Gluconobacter oxydans
n-butanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
1-pentanol + acceptor
strain DSM 4025, 49.8% of activity with 1-propanol
287728
Gluconobacter oxydans
n-pentanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
1-propanol + acceptor
-
287728
Gluconobacter oxydans
n-propanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
2-butanol + acceptor
strain DSM 4025, 87.4% of activity with 1-propanol
287728
Gluconobacter oxydans
2-butanone + reduced acceptor
-
287728
Gluconobacter oxydans
?
2-propanol + acceptor
strain DSM 4025, 78.2% of activity with 1-propanol
287728
Gluconobacter oxydans
acetone + reduced acceptor
-
287728
Gluconobacter oxydans
?
D-fructose + acceptor
strain DSM 4025, 6.6% of activity with 1-propanol
287728
Gluconobacter oxydans
2-keto-D-gulonate + reduced acceptor
-
287728
Gluconobacter oxydans
?
D-glucose + acceptor
strain DSM 4025, 25.3% of activity with 1-propanol
287728
Gluconobacter oxydans
D-gluconic acid + reduced acceptor
-
287728
Gluconobacter oxydans
?
D-mannitol + acceptor
strain DSM 4025, 16.2% of activity with 1-propanol
287728
Gluconobacter oxydans
? + reduced acceptor
-
287728
Gluconobacter oxydans
?
D-sorbitol + acceptor
strain DSM 4025, 7.1% of activity with 1-propanol
287728
Gluconobacter oxydans
D-glucose + D-gluconic acid + L-sorbose + reduced acceptor
-
287728
Gluconobacter oxydans
?
DL-glyceraldehyde + acceptor
strain DSM 4025, 80.2% of activity with 1-propanol
287728
Gluconobacter oxydans
? + reduced acceptor
-
287728
Gluconobacter oxydans
?
ethanol + acceptor
strain DSM 4025, 88.6% of activity with 1-propanol
287728
Gluconobacter oxydans
ethanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
L-sorbose + acceptor
strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol
287728
Gluconobacter oxydans
L-sorbosone + reduced acceptor
-
-
-
?
L-sorbose + acceptor
strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol
287728
Gluconobacter oxydans
L-sorbosone + reduced acceptor
-
287728
Gluconobacter oxydans
?
L-sorbosone + acceptor
strain DSM 4025, 16.2% of activity with 1-propanol
287728
Gluconobacter oxydans
2-keto-L-gulonate + reduced acceptor
-
287728
Gluconobacter oxydans
?
n-heptanol + acceptor
strain DSM 4025, 47.7% of activity with 1-propanol
287728
Gluconobacter oxydans
n-heptanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
n-hexanol + acceptor
strain DSM 4025, 49.1% of activity with 1-propanol
287728
Gluconobacter oxydans
n-hexanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
polyethylene glycol 100 + acceptor
strain DSM 4025, 68.9% of activity with 1-propanol
287728
Gluconobacter oxydans
?
-
-
-
?
polyvinyl alcohol + acceptor
strain DSM 4025, 38.1% of activity with 1-propanol
287728
Gluconobacter oxydans
oxidized polyvinyl alcohol + reduced acceptor
-
287728
Gluconobacter oxydans
?
propionaldehyde + acceptor
strain DSM 4025, 83.3% of activity with 1-propanol
287728
Gluconobacter oxydans
propanoate + reduced acceptor
-
287728
Gluconobacter oxydans
?
Subunits
Subunits
Commentary
Organism
dimer
strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
40
strain DSM 4025
Gluconobacter oxydans
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
50
strain DSM 4025
Gluconobacter oxydans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
strain DSM 4025, enzyme is stable at and below 30°C, but unstable above 40°C
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
strain DSM 4025
Gluconobacter oxydans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
strain DSM 4025, enzyme is stable in alkaline pH and unstable with increasing acidity
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome c
strain DSM 4025, physiological electron acceptor
Gluconobacter oxydans
pyrroloquinoline quinone
strain DSM 4025, non-covalently bound, 2 mol PQQ/mol enzyme
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome c
strain DSM 4025, physiological electron acceptor
Gluconobacter oxydans
pyrroloquinoline quinone
strain DSM 4025, non-covalently bound, 2 mol PQQ/mol enzyme
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CoCl2
strain DSM 4025, 5 mM, 84% inhibition
Gluconobacter oxydans
EDTA
strain DSM 4025, 5 mM, 87% inhibition
Gluconobacter oxydans
EGTA
strain DSM 4025, 5 mM, 89% inhibition
Gluconobacter oxydans
FeCl2
strain DSM 4025, 5 mM, 89% inhibition
Gluconobacter oxydans
FeCl3
strain DSM 4025, 5 mM, 97% inhibition
Gluconobacter oxydans
NiSO4
strain DSM 4025, 5 mM, 77% inhibition
Gluconobacter oxydans
ZnCl2
strain DSM 4025, 5 mM, 90% inhibition
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
1-propanol
strain DSM 4025
Gluconobacter oxydans
230
-
L-sorbose
strain DSM 4025
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62500
-
strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
Gluconobacter oxydans
64500
-
strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
Gluconobacter oxydans
135000
-
strain DSM 4025, gel filtration
Gluconobacter oxydans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-sorbose + acceptor
Gluconobacter oxydans
strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol
L-sorbosone + reduced acceptor
-
Gluconobacter oxydans
?
Purification (Commentary) (protein specific)
Commentary
Organism
strain DSM 4025, PEG 600, DEAE-Toyopearl, Q-Sepharose
Gluconobacter oxydans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.73
-
strain DSM 4025
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + acceptor
strain DSM 4025, 69.4% of activity with 1-propanol
287728
Gluconobacter oxydans
n-butanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
1-pentanol + acceptor
strain DSM 4025, 49.8% of activity with 1-propanol
287728
Gluconobacter oxydans
n-pentanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
1-propanol + acceptor
-
287728
Gluconobacter oxydans
n-propanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
2-butanol + acceptor
strain DSM 4025, 87.4% of activity with 1-propanol
287728
Gluconobacter oxydans
2-butanone + reduced acceptor
-
287728
Gluconobacter oxydans
?
2-propanol + acceptor
strain DSM 4025, 78.2% of activity with 1-propanol
287728
Gluconobacter oxydans
acetone + reduced acceptor
-
287728
Gluconobacter oxydans
?
D-fructose + acceptor
strain DSM 4025, 6.6% of activity with 1-propanol
287728
Gluconobacter oxydans
2-keto-D-gulonate + reduced acceptor
-
287728
Gluconobacter oxydans
?
D-glucose + acceptor
strain DSM 4025, 25.3% of activity with 1-propanol
287728
Gluconobacter oxydans
D-gluconic acid + reduced acceptor
-
287728
Gluconobacter oxydans
?
D-mannitol + acceptor
strain DSM 4025, 16.2% of activity with 1-propanol
287728
Gluconobacter oxydans
? + reduced acceptor
-
287728
Gluconobacter oxydans
?
D-sorbitol + acceptor
strain DSM 4025, 7.1% of activity with 1-propanol
287728
Gluconobacter oxydans
D-glucose + D-gluconic acid + L-sorbose + reduced acceptor
-
287728
Gluconobacter oxydans
?
DL-glyceraldehyde + acceptor
strain DSM 4025, 80.2% of activity with 1-propanol
287728
Gluconobacter oxydans
? + reduced acceptor
-
287728
Gluconobacter oxydans
?
ethanol + acceptor
strain DSM 4025, 88.6% of activity with 1-propanol
287728
Gluconobacter oxydans
ethanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
L-sorbose + acceptor
strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol
287728
Gluconobacter oxydans
L-sorbosone + reduced acceptor
-
-
-
?
L-sorbose + acceptor
strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol
287728
Gluconobacter oxydans
L-sorbosone + reduced acceptor
-
287728
Gluconobacter oxydans
?
L-sorbosone + acceptor
strain DSM 4025, 16.2% of activity with 1-propanol
287728
Gluconobacter oxydans
2-keto-L-gulonate + reduced acceptor
-
287728
Gluconobacter oxydans
?
n-heptanol + acceptor
strain DSM 4025, 47.7% of activity with 1-propanol
287728
Gluconobacter oxydans
n-heptanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
n-hexanol + acceptor
strain DSM 4025, 49.1% of activity with 1-propanol
287728
Gluconobacter oxydans
n-hexanal + reduced acceptor
-
287728
Gluconobacter oxydans
?
polyethylene glycol 100 + acceptor
strain DSM 4025, 68.9% of activity with 1-propanol
287728
Gluconobacter oxydans
?
-
-
-
?
polyvinyl alcohol + acceptor
strain DSM 4025, 38.1% of activity with 1-propanol
287728
Gluconobacter oxydans
oxidized polyvinyl alcohol + reduced acceptor
-
287728
Gluconobacter oxydans
?
propionaldehyde + acceptor
strain DSM 4025, 83.3% of activity with 1-propanol
287728
Gluconobacter oxydans
propanoate + reduced acceptor
-
287728
Gluconobacter oxydans
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
40
strain DSM 4025
Gluconobacter oxydans
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
50
strain DSM 4025
Gluconobacter oxydans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
strain DSM 4025, enzyme is stable at and below 30°C, but unstable above 40°C
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
9
strain DSM 4025
Gluconobacter oxydans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
strain DSM 4025, enzyme is stable in alkaline pH and unstable with increasing acidity
Gluconobacter oxydans
Other publictions for EC 1.1.99.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740005
Wang
-
Overexpression of pyrroloquino ...
Gluconobacter oxydans, Gluconobacter oxydans WSH-003
Biochem. Eng. J.
112
70-77
2016
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1
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740828
Hu
Enhanced production of L-sorbo ...
Gluconobacter oxydans, Gluconobacter oxydans WSH-003
J. Ind. Microbiol. Biotechnol.
42
1039-1047
2015
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725870
Du
Combinational expression of so ...
Ketogulonicigenium vulgare, Ketogulonicigenium vulgare HB602
Metab. Eng.
19C
50-56
2013
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1
1
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724570
Zhu
Sporulation and spore stabilit ...
Ketogulonicigenium vulgare
Biores. Technol.
107
399-404
2012
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5
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725582
Cai
Genetic engineering of Ketogul ...
Ketogulonicigenium vulgare, Ketogulonicigenium vulgare DSM 4025
J. Biotechnol.
157
320-325
2012
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684602
Fu
Identification of promoters of ...
Escherichia coli, Ketogulonicigenium vulgare
Appl. Microbiol. Biotechnol.
75
1127-1132
2007
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2
2
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5
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2
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2
2
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1
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673382
Zhang
-
Purification and characterizat ...
Gluconobacter oxydans
Enzyme Microb. Technol.
38
643-648
2006
1
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4
1
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1
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1
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1
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1
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1
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4
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1
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1
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1
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1
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1
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657424
Hao
-
Properties and functions of L- ...
Gluconobacter oxydans
Yingyong Yu Huanjing Shengwu Xuebao
8
204-208
2002
1
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1
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2
1
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1
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1
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1
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1
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1
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1
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1
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-
287728
Asakura
Isolation and Characterization ...
Gluconobacter oxydans
Biosci. Biotechnol. Biochem.
63
46-53
1999
-
-
-
-
-
-
7
2
-
-
3
1
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1
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