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Literature summary for 1.1.5.9 extracted from

  • Sode, K.; Tsugawa, W.; Yamazaki, T.; Watanabe, M.; Ogasawara, N.; Tanaka, M.
    A novel thermostable glucose dehydrogenase varying temperature properties by altering its quaternary structures (1996), Enzyme Microb. Technol., 19, 82-85.
No PubMed abstract available

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Burkholderia cepacia 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
after preincubation at temperatures below 45┬░C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
67000
-
after preincubation at 70┬░C for 30 min: x * 67000 (alpha-subunit), SDS-PAGE Burkholderia cepacia
67000
-
after preincubation at temperatures below 45┬░C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
85000
-
after preincubation at 70┬░C for 30 min, native PAGE Burkholderia cepacia
350000
-
native PAGE Burkholderia cepacia
380000
-
gel filtration chromatography Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
bacterial strain SM4 is used, isolated from a soil sample near a Japanese hot spring
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
6
-
at 75┬░C after preincubation of the enzyme at 70┬░C for 30 min Burkholderia cepacia
15
-
at 70┬░C Burkholderia cepacia
45
-
at 45┬░C Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
heteromer after preincubation at temperatures below 45┬░C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE Burkholderia cepacia
homooligomer after preincubation at 70┬░C for 30 min: x * 67000 (alpha-subunit), SDS-PAGE Burkholderia cepacia

Synonyms

Synonyms Comment Organism
thermostable GDH
-
Burkholderia cepacia
thermostable glucose dehydrogenase
-
Burkholderia cepacia

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
45
-
enzyme activity shows a second peak at 70┬░C with 30% of maximal activity Burkholderia cepacia
75
-
after preincubating the enzyme at 70┬░C for 30 min Burkholderia cepacia

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
20 85 20% of maximal activity at 20┬░C and 10% of maximal activity at 85┬░C, maximal activity is at 45┬░C with a second activity peak at 70┬░C (30% of maximal activity) Burkholderia cepacia
40 85 after preincubation of the enzyme at 70┬░C for 30 min: 15% of maximal activity at 40┬░C and 50% of maximal activity at 85┬░C, maximal activity at 75┬░C Burkholderia cepacia

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
5 70 enzyme is stable at temperatures between 5-37┬░C for 30 min, after incubating enzyme at 45┬░C for 30 min 40% of enzyme activity remains, at 70┬░C for 30 min 10% of enzyme activity remains Burkholderia cepacia
5 75 after preincubation at 70┬░C for 30 min the enzyme is stable at temperatures between 5-50┬░C for 30 min, after incubating enzyme at 70┬░C for 30 min 70% of activity remains Burkholderia cepacia