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Literature summary for 1.1.5.9 extracted from

  • Matsushita, K.; Ohno, Y.; Shinagawa, E.; Adachi, O.; Ameyama, M.
    Gluconsäure bildende Enzyme bei Aspergillus niger (1980), Agric. Biol. Chem., 44, 1505-1512.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
p-benzoquinone competitive inhibition of the activity with 2,6-dichlorophenolindophenol or coenzyme Q1, non-competitive inhibition of reaction with phenazine methosulfate Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
coenzyme Q1
-
Pseudomonas sp.
0.13
-
phenazine methosulfate
-
Pseudomonas sp.
0.47
-
D-glucose reaction with 2,6-dichlorophenolindophenol at pH 6.0 Pseudomonas sp.
0.56
-
tetramethyl-p-phenylenediamine
-
Pseudomonas sp.
0.69
-
ferricyanide
-
Pseudomonas sp.
1.6
-
2,6-dichlorophenolindophenol
-
Pseudomonas sp.
6.3
-
D-glucose reaction with phenazine methosulfate/2,6-dichlorophenolindophenol at pH 8.75 Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane bound to Pseudomonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
1 * 87000, monomeric in presence of 1% Triton X-100, aggregation after removing the detergent, urea-SDS-PAGE Pseudomonas sp.
93000
-
sucrose density gradient centrifugation, in presence of Triton X-100 Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + acceptor 6.5% of the activity with D-glucose Pseudomonas sp. D-galactono-1,5-lactone + reduced acceptor
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Pseudomonas sp. D-gluconic acid + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + coenzyme Q1
-
Pseudomonas sp. D-glucono-1,5-lactone + reduced coenzyme Q1
-
?
D-glucose + ferricyanide
-
Pseudomonas sp. D-glucono-1,5-lactone + ferrocyanide
-
?
D-glucose + phenazine methosulfate
-
Pseudomonas sp. D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
D-glucose + tetramethyl-p-phenylenediamine
-
Pseudomonas sp. D-glucono-1,5-lactone + reduced tetramethyl-p-phenylenediamine
-
?
D-mannose + acceptor 8.6% of the activity with D-glucose Pseudomonas sp. D-manno-1,5-lactone + reduced acceptor
-
?
D-xylose + acceptor 13% of the activity with D-glucose Pseudomonas sp. D-xylono-1,5-lactone + reduced acceptor
-
?
L-arabinose + acceptor 2.8% of the activity with D-glucose Pseudomonas sp. L-arabinono-1,5-lactone + reduced acceptor
-
?
L-rhamnose + acceptor 7.5% of the activity with D-glucose Pseudomonas sp. L-rhamnone-1,5-lactone + reduced acceptor
-
?
maltose + acceptor 3.2% of the activity with D-glucose Pseudomonas sp. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 87000, monomeric in presence of 1% Triton X-100, aggregation after removing the detergent, urea-SDS-PAGE Pseudomonas sp.
More aggregation in medium containing no Triton X-100 to dimers, trimers and tetramers Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
reaction with ferricyanide Pseudomonas sp.
6
-
reaction with 2,6-dichlorophenolindophenol Pseudomonas sp.
6.5
-
reaction with coenzyme Q1 Pseudomonas sp.
8.8
-
reaction with phenazine methosulfate or tetramethyl-p-phenylenediamine Pseudomonas sp.