Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pleurotus eryngii |
Protein Variants | Comment | Organism |
---|---|---|
H502S | the mutant shows much lower activities on 4-nitrobenzaldehyde (340fold activity decrease) than the wild type enzyme | Pleurotus eryngii |
H546S | the mutant shows much lower activities on 4-nitrobenzaldehyde (670fold activity decrease) than the wild type enzyme | Pleurotus eryngii |
Y92F | the mutation causes a 5fold reduction in the p-anisaldehyde kcat value | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
4-anisaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.7 | - |
3-chloro-4-anisaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.8 | - |
4-anisaldehyde | mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
1.5 | - |
3-Chlorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
2 | - |
4-nitrobenzaldehyde | mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
2.2 | - |
3-Fluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
3 | - |
3,4-difluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
4.7 | - |
4-Chlorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
4.9 | - |
4-Fluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
5 | - |
4-nitrobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
7 | - |
benzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
8 | - |
veratraldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
13 | - |
2,4-hexadienal | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94219 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-hexadien-1-ol + O2 | - |
Pleurotus eryngii | 2,4-hexadienal + H2O2 | - |
? | |
2,4-hexadienal + O2 | - |
Pleurotus eryngii | 2,4-hexadienoate + H2O2 | - |
? | |
3,4-difluorobenzaldehyde + O2 | - |
Pleurotus eryngii | 3,4-difluorobenzoic acid + H2O2 | - |
? | |
3,4-difluorobenzaldehyde + O2 | - |
Pleurotus eryngii | 3,4-difluorobenzoic acid + H2O2 | - |
r | |
3-chloro-4-anisaldehyde + O2 | - |
Pleurotus eryngii | 3-chloro-4-anisic acid + H2O2 | - |
r | |
3-chloro-4-anisyl alcohol + O2 | - |
Pleurotus eryngii | 3-chloro-4-anisaldehyde + H2O2 | - |
? | |
3-chlorobenzaldehyde + O2 | - |
Pleurotus eryngii | 3-chlorobenzoic acid + H2O2 | - |
r | |
3-chlorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 3-chlorobenzaldehyde + H2O2 | - |
? | |
3-fluorobenzaldehyde + O2 | - |
Pleurotus eryngii | 3-fluorobenzoic acid + H2O2 | - |
r | |
3-fluorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 3-fluorobenzaldehyde + H2O2 | - |
? | |
4-anisaldehyde + O2 | - |
Pleurotus eryngii | 4-anisic acid + H2O2 | - |
r | |
4-anisyl alcohol + O2 | - |
Pleurotus eryngii | 4-anisaldehyde + H2O2 | - |
? | |
4-chlorobenzaldehyde + O2 | - |
Pleurotus eryngii | 4-chlorobenzoic acid + H2O2 | - |
r | |
4-chlorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-chlorobenzaldehyde + H2O2 | - |
? | |
4-fluorobenzaldehyde + O2 | - |
Pleurotus eryngii | 4-fluorobenzoic acid + H2O2 | - |
r | |
4-fluorobenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-fluorobenzaldehyde + H2O2 | - |
? | |
4-nitrobenzaldehyde + O2 | - |
Pleurotus eryngii | 4-nitrobenzoic acid + H2O2 | - |
? | |
4-nitrobenzaldehyde + O2 | - |
Pleurotus eryngii | 4-nitrobenzoic acid + H2O2 | - |
r | |
benzaldehyde + O2 | - |
Pleurotus eryngii | benzoic acid + H2O2 | - |
r | |
benzyl alcohol + O2 | - |
Pleurotus eryngii | benzaldehyde + H2O2 | - |
? | |
additional information | AAO typically oxidizes aromatic alcohols to the corresponding aldehydes. However, the enzyme can also oxidize aromatic aldehydes to the corresponding acids | Pleurotus eryngii | ? | - |
? | |
veratraldehyde + O2 | - |
Pleurotus eryngii | veratric acid + H2O2 | - |
r | |
veratryl alcohol + O2 | - |
Pleurotus eryngii | veratraldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAO | - |
Pleurotus eryngii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
4-anisaldehyde | mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.05 | - |
4-anisaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.057 | - |
3-chloro-4-anisaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.13 | - |
veratraldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.33 | - |
2,4-hexadienal | wild type enzyme, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.367 | - |
4-Fluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.5 | - |
benzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.85 | - |
3-Chlorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.867 | - |
3,4-difluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.883 | - |
3-Fluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
1.05 | - |
4-Chlorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
1.21 | - |
4-nitrobenzaldehyde | mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
1.633 | - |
4-nitrobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pleurotus eryngii |
General Information | Comment | Organism |
---|---|---|
physiological function | aryl-alcohol oxidase provides H2O2 for lignin biodegradation | Pleurotus eryngii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
4-anisaldehyde | mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.0167 | - |
veratraldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.025 | - |
2,4-hexadienal | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.073 | - |
benzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.075 | - |
4-Fluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.085 | - |
3-chloro-4-anisaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.087 | - |
4-anisaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.223 | - |
4-Chlorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.282 | - |
3,4-difluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.315 | - |
4-nitrobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.407 | - |
3-Fluorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.597 | - |
4-nitrobenzaldehyde | mutant enzyme Y92F, wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii | |
0.643 | - |
3-Chlorobenzaldehyde | wild type enzyme, at 24°C, 0.1 M sodium phosphate buffer, pH 6.0 | Pleurotus eryngii |