KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
26-Hydroxycholesterol | 5-androstene-3beta,17beta-diol | Nocardia erythropolis | |
0.0014 | - |
25-Hydroxy-27-norcholesterol | - |
Nocardia erythropolis | |
0.0023 | - |
5alpha-Cholestan-3beta-ol | - |
Nocardia erythropolis | |
0.0028 | - |
5alpha-Cholest-8(14)-en-3beta-ol | - |
Nocardia erythropolis | |
0.0029 | - |
cholesterol | - |
Nocardia erythropolis | |
0.0031 | - |
17beta-Hydroxymethyl-5-androsten-3beta-ol | - |
Nocardia erythropolis | |
0.0037 | - |
5-pregnene-3beta,20beta-diol | - |
Nocardia erythropolis | |
0.0048 | - |
5-Cholene-3beta,24-diol | - |
Nocardia erythropolis | |
0.0055 | - |
5,7-cholestadien-3beta-ol | - |
Nocardia erythropolis | |
0.0075 | - |
4,4-Dimethylcholesterol | - |
Nocardia erythropolis | |
0.0093 | - |
23,24-dinor-5-cholene-3beta,22-diol | - |
Nocardia erythropolis | |
0.0107 | - |
5alpha-cholest-8(14)en-3beta-ol | - |
Nocardia erythropolis | |
0.0126 | - |
4alpha-Methylcholesterol | - |
Nocardia erythropolis | |
0.0284 | - |
4beta-methylcholesterol | - |
Nocardia erythropolis | |
0.124 | - |
20,25-Diazacholesterol | - |
Nocardia erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Nocardia erythropolis | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia erythropolis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Nocardia erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-hydroxymethyl-5-androsten-3beta-ol + O2 | - |
Nocardia erythropolis | 17beta-hydroxymethyl-5-androsten-3-one + H2O2 | - |
? | |
20,25-diazacholesterol + O2 | - |
Nocardia erythropolis | 20,25-diazacholest-4-en-3-one + H2O2 | - |
? | |
23,24-dinor-5-cholen-3beta,22-diol + O2 | - |
Nocardia erythropolis | 23,24-dinor-5-cholen-22-ol-3-one + H2O2 | - |
? | |
23,24-dinor-5-cholene-3beta,22-diol + O2 | - |
Nocardia erythropolis | 23,24-dinor-5-cholen-22-ol-3-one + H2O2 | - |
r | |
25-hydroxy-27-norcholesterol + O2 | - |
Nocardia erythropolis | ? | - |
? | |
26-hydroxycholesterol + O2 | - |
Nocardia erythropolis | 26-hydroxycholest-5-en-3-one + H2O2 | - |
? | |
4,4-dimethylcholesterol + O2 | - |
Nocardia erythropolis | 4,4-dimethylcholest-5-en-3-one | - |
? | |
4alpha-methylcholesterol + O2 | - |
Nocardia erythropolis | 4alpha-methylcholest-5-en-3-one + H2O2 | - |
? | |
4beta-methylcholesterol + O2 | - |
Nocardia erythropolis | 4beta-methylcholest-5-en-3-one + H2O2 | - |
? | |
5,7-cholestadien-3beta-ol + O2 | - |
Nocardia erythropolis | 5,7-cholestadien-3-one + H2O2 | - |
? | |
5-androstene-3beta,17beta-diol + O2 | - |
Nocardia erythropolis | 5-androsten-17beta-ol-3-one + H2O2 | - |
? | |
5-cholene-3beta,24-diol + O2 | - |
Nocardia erythropolis | 5-cholen-24-ol-3-one + H2O2 | - |
? | |
5-pregnen-3beta,20beta-diol + O2 | - |
Nocardia erythropolis | 5-pregnen-20beta-ol-3-one + H2O2 | - |
? | |
5alpha-cholest-8(14)-en-3beta-ol + O2 | - |
Nocardia erythropolis | 5alpha-cholest-8(14)-en-3-one + H2O2 | - |
? | |
5alpha-cholestan-3beta-ol + O2 | - |
Nocardia erythropolis | 5alpha-cholestan-3-one + H2O2 | - |
? | |
cholesterol + O2 | enzyme specifically oxidizes 3beta-hydroxy groups in steroids | Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Nocardia erythropolis | cholest-5-en-3-one + H2O2 | - |
? |