Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain SMD1168 | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.95 | - |
beta-D-glucose | recombinant enzyme, in 0.1 M sodium phosphate buffer, pH 6.0, at 35°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | P13006 | - |
- |
Aspergillus niger Z-25 | P13006 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
153.5 | - |
recombinant enzyme after 3.34fold purification, at 35°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 + H2O | - |
Aspergillus niger Z-25 | D-glucono-1,5-lactone + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucose: oxygen 1-oxidoreductase | - |
Aspergillus niger |
GOD | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | the activity of recombinant GOD increases slightly at the reaction temperature ranging from 20-40°C, and then falls moderately till 60°C. The recombinant enzyme retains more than 90% of activity within 40°C. Over 30% of activity is lost at 50°C, whereas almost no activity is detected above 60°C | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
484.3 | - |
beta-D-glucose | recombinant enzyme, in 0.1 M sodium phosphate buffer, pH 6.0, at 35°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | more than 88% of the maximum activity is observed between pH 4.0 and 8.0, outside this range the activity decreases dramatically | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Aspergillus niger |