Application | Comment | Organism |
---|---|---|
analysis | development of a screening assay for the substrate specificity of para-phenol oxidases based on the detection of hydrogen peroxide using the ferric-xylenol orange complex method | Penicillium simplicissimum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Penicillium simplicissimum |
Protein Variants | Comment | Organism |
---|---|---|
C470L | mutant displays similar activity to the wild-type enzyme with the substrates vanillyl alcohol, chavicol aund eugenol, but no activity with linear 4-alkylphenols | Penicillium simplicissimum |
F424G | mutant does not contain any flavin after purification | Penicillium simplicissimum |
I468V | mutant displays similar activity to the wild-type enzyme with the substrates vanillyl alcohol, chavicol aund eugenol, but no activity with linear 4-alkylphenols | Penicillium simplicissimum |
L316M | mutant displays substrate specificity profile similar to wild-type | Penicillium simplicissimum |
T457Q | mutant shows about 3fold increased activity towards vanillyl alcohol, but decrease in activity with all other substrates | Penicillium simplicissimum |
T459I | mutant displays substrate specificity profile similar to wild-type | Penicillium simplicissimum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.129 | - |
Vanillyl alcohol | His-tagged recombinant enzyme, pH 7.5, 25°C | Penicillium simplicissimum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium simplicissimum | P56216 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-cyclohexylphenol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
4-cyclopentylphenol + O2 | - |
Penicillium simplicissimum | 4-(1-cyclopenten-1-yl)phenol | - |
? | |
4-ethylphenol + O2 + H2O | - |
Penicillium simplicissimum | 1-(4'-hydroxyphenyl)ethanol + H2O2 | - |
? | |
4-n-propylphenol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
chavicol + O2 + H2O | - |
Penicillium simplicissimum | coumaryl alcohol + H2O2 | - |
? | |
eugenol + O2 + H2O | - |
Penicillium simplicissimum | coniferyl alcohol + H2O2 | - |
? | |
vanillyl alcohol + O2 | - |
Penicillium simplicissimum | vanillin + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
Vanillyl alcohol | His-tagged recombinant enzyme, pH 7.5, 25°C | Penicillium simplicissimum | |
3.7 | - |
4-cyclopentylphenol | His-tagged recombinant enzyme, pH 7.5, 25°C | Penicillium simplicissimum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
Vanillyl alcohol | His-tagged recombinant enzyme, pH 7.5, 25°C | Penicillium simplicissimum |