Crystallization (Comment) | Organism |
---|---|
crystals of mutant enzyme D170S are grown using hanging-drop vapor diffusion method | Penicillium simplicissimum |
Protein Variants | Comment | Organism |
---|---|---|
D170A | 3100fold decrease in turnover-number for for 4-(methoxymethyl)phenol | Penicillium simplicissimum |
D170E | 2fold increase in KM-value for for 4-(methoxymethyl)phenol, 2.1fold increase in Km-value for vanillyl alcohol, 2fold decrease in KM-value for eugenol as compared to wild-type enzyme.442fold decrease in turnover-number for for 4-(methoxymethyl)phenol, 2.5fold decrease in turnover-number for vanillyl alcohol, 51.5fold decrease in turnover-number for eugenol as compared to wild-type enzyme.Redox potential of mutant enzyme, + 6 mV, is decreased compared to wild-type enzyme, 55 mV | Penicillium simplicissimum |
D170N | 1550fold decrease in turnover-number for for 4-(methoxymethyl)phenol | Penicillium simplicissimum |
D170S | 2fold decrease in KM-value for eugenol as compared to wild-type enzyme.1290fold decrease in turnover-number for for 4-(methoxymethyl)phenol, 825fold decrease in turnover-number for vanillyl alcohol, 1750fold decrease in turnover-number for eugenol as compared to wild-type enzyme. Redox potential of mutant enzyme, -91 mV, is decreased compared to wild-type enzyme, 55 mV | Penicillium simplicissimum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
eugenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.002 | - |
eugenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.113 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.34 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium simplicissimum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Penicillium simplicissimum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(methoxymethyl)phenol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
eugenol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
vanillyl alcohol + O2 | - |
Penicillium simplicissimum | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme D170N | Penicillium simplicissimum | |
0.004 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.004 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.01 | - |
eugenol | pH 7.5, 25°C, mutant enzyme D170S | Penicillium simplicissimum | |
0.07 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
0.34 | - |
eugenol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum | |
1.3 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme D170E | Penicillium simplicissimum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Penicillium simplicissimum |