Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aerococcus viridans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
(S)-lactate | pH 7.0, 4°C | Aerococcus viridans | |
0.00094 | - |
(S)-lactate | pH 7.0, 25°C | Aerococcus viridans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Aerococcus viridans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aerococcus viridans | Q44467 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
210 | - |
pH 7.0, 37°C | Aerococcus viridans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + O2 | - |
Aerococcus viridans | pyruvate + H2O2 | - |
? | |
additional information | contrary to lactate monooxygenase, with lactate oxidase the complex of reduced flavin enzyme and pyruvate dissociates rapidly, with the result that it is the free reduced flavin form of the enzyme that reacts with O2, to give the observed products, pyruvate and H2O2 | Aerococcus viridans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE and calculated | Aerococcus viridans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31 | - |
(S)-lactate | pH 7.0, 4°C | Aerococcus viridans | |
280 | - |
(S)-lactate | pH 7.0, 25°C | Aerococcus viridans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Aerococcus viridans |