Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-2-hydroxy-3-butynoate | irreversible inactivation after 25 turnovers, covalent addition to the coenzyme | Rattus sp. | |
DL-2-hydroxy-3-heptynoate | inactivation after18000 turnovers | Rattus sp. | |
DL-2-hydroxy-3-hexynoate | inactivation after 8500 turnovers | Rattus sp. | |
DL-2-hydroxy-3-octynoate | inactivation after 15000 turnovers | Rattus sp. | |
DL-2-hydroxy-3-pentynoate | inactivation after 4800 turnovers | Rattus sp. | |
DL-vinylglycolate | slight inactivation after 10000 turnovers | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
DL-2-hydroxy-3-octynoate | - |
Rattus sp. | |
0.38 | - |
DL-2-hydroxy-3-heptynoate | - |
Rattus sp. | |
4 | - |
DL-2-hydroxy-3-butynoate | - |
Rattus sp. | |
7 | - |
DL-2-hydroxy-3-hexynoate | - |
Rattus sp. | |
9 | - |
DL-2-hydroxy-3-pentynoate | - |
Rattus sp. | |
10 | - |
DL-vinylglycolate | - |
Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxybutyrate + O2 | Rattus sp. | - |
2-oxobutyrate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-2-hydroxy-3-butynoate + O2 | good substrate, but inactivation after 25 turnovers | Rattus sp. | 2-oxo-3-butynoate + H2O2 | - |
? | |
DL-2-hydroxy-3-heptynoate + O2 | 86% of the activity compared to DL-2-hydroxybutyrate | Rattus sp. | 2-oxo-3-heptynoate + H2O2 | - |
? | |
DL-2-hydroxy-3-hexynoate + O2 | 65% of the activity compared to DL-2-hydroxybutyrate | Rattus sp. | 2-oxo-3-hexynoate + H2O2 | - |
? | |
DL-2-hydroxy-3-octynoate + O2 | 70% of the activity compared to DL-2-hydroxybutyrate | Rattus sp. | 2-oxo-3-octynoate + H2O2 | - |
? | |
DL-2-hydroxy-3-pentynoate + O2 | 2fold higher activity compared to DL-2-hydroxybutyrate | Rattus sp. | 2-oxo-3-pentynoate + H2O2 | - |
? | |
DL-2-hydroxybutyrate + O2 | - |
Rattus sp. | 2-oxobutyrate + H2O2 | - |
? | |
DL-3-chlorolactate + O2 | - |
Rattus sp. | 3-chloropyruvate + H2O2 | - |
? | |
DL-vinylglycolate + O2 | 90% of the activity compared to DL-2-hydroxybutyrate | Rattus sp. | 2-oxo-3-butenoic acid + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Rattus sp. |