Crystallization (Comment) | Organism |
---|---|
- |
Pichia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 10% inhibition | Pichia sp. | |
2,2'-dipyridyl | 1 mM, 13% inhibition | Pichia sp. | |
2-Aminoethanol | 1 mM, 70% inhibition | Pichia sp. | |
3-Pentanol | 1 mM, 16% inhibition | Pichia sp. | |
5,5'-dithiobis(2-nitrobenzoate) | 1 mM, 35% inhibition | Pichia sp. | |
8-hydroxyquinoline | 1 mM, 6% inhibition | Pichia sp. | |
acetamide | 1 mM, 10% inhibition | Pichia sp. | |
Cu2+ | 1 mM CuSO4, 50% inhibition | Pichia sp. | |
H2O2 | - |
Pichia sp. | |
hydroxylamine | 1 mM, 21% inhibition | Pichia sp. | |
imidazole | 1 mM, 20% inhibition | Pichia sp. | |
iodoacetate | 1 mM, 38% inhibition | Pichia sp. | |
Mercuric chloride | 1 mM, complete inhibition | Pichia sp. | |
metal chelators | slight | Pichia sp. | |
NEM | 1 mM, 10% inhibition | Pichia sp. | |
p-hydroxymercuribenzoate | 1 mM, complete inhibition | Pichia sp. | |
PCMB | - |
Pichia sp. | |
Sodium azide | 1 mM, 15% inhibition | Pichia sp. | |
Thiosemicarbazide | 1 mM, 31% loss of activity | Pichia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
methanol | - |
Pichia sp. | |
3.5 | - |
formaldehyde | - |
Pichia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
4 * 76000, SDS-PAGE | Pichia sp. |
300000 | - |
gel filtration | Pichia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + O2 | Pichia sp. | key enzyme of methanol metabolism | formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pichia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pichia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Pichia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pentanol + O2 | 30% of the activity with methanol | Pichia sp. | n-pentanal + H2O2 | - |
? | |
2-mercaptoethanol + O2 | 25% of the activity with methanol | Pichia sp. | 2-mercaptoethanal + H2O2 | - |
? | |
2-methyl-1-butanol + O2 | 22% of the activity with methanol | Pichia sp. | 2-methyl-1-butanal + H2O2 | - |
? | |
3-chloro-1-propanol + O2 | 22% of the activity with methanol | Pichia sp. | 3-chloro-1-propanal + H2O2 | - |
? | |
4-chloro-1-butanol + O2 | 11% of the activity with methanol | Pichia sp. | 4-chloro-1-butanal + H2O2 | - |
? | |
chloroethanol + O2 | 2-chloroethanol, 70% of the activity with methanol | Pichia sp. | chloroethanal + H2O2 | - |
? | |
ethanol + O2 | 92% of the activity with methanol | Pichia sp. | acetaldehyde + H2O2 | - |
? | |
ethylene glycol mono-methylether + O2 | - |
Pichia sp. | methoxyacetaldehyde + H2O2 | - |
? | |
formaldehyde + O2 | 15% of the activity with methanol | Pichia sp. | formate + H2O2 | - |
? | |
isobutanol + O2 | 2% of the activity with methanol | Pichia sp. | isobutanal + H2O2 | - |
? | |
methanol + O2 | - |
Pichia sp. | formaldehyde + H2O2 | - |
? | |
methanol + O2 | key enzyme of methanol metabolism | Pichia sp. | formaldehyde + H2O2 | - |
? | |
n-butanol + O2 | 52%, of the activity with methanol | Pichia sp. | n-butanal + H2O2 | - |
? | |
n-hexanol + O2 | 4% of the activity with methanol | Pichia sp. | n-hexanal + H2O2 | - |
? | |
n-propanol + O2 | - |
Pichia sp. | n-propanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 76000, SDS-PAGE | Pichia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pichia sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 65% of maximal activity, pH 10.0: 85% of maximal activity | Pichia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pichia sp. |