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Literature summary for 1.1.3.10 extracted from

  • Pisanelli, I.; Kujawa, M.; Spadiut, O.; Kittl, R.; Halada, P.; Volc, J.; Mozuch, M.D.; Kersten, P.; Haltrich, D.; Peterbauer, C.
    Pyranose 2-oxidase from Phanerochaete chrysosporium--expression in E. coli and biochemical characterization (2009), J. Biotechnol., 142, 97-106.
    View publication on PubMed
Search for more literature for 1.1.3.10

Cloned(Commentary)

Cloned (Comment) Organism
heterologously expressed in Escherichia coli DH5 alpha as His-tagged protein Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 1,4-benzoquinone substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 4.5 Phanerodontia chrysosporium
0.051
-
 2,6-dichloroindophenol substrate 2,6-dichloroindophenol (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.09
-
 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical substrate 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.11
-
 1,4-benzoquinone substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.21
-
 2-methoxy-1,4-benzoquinone substrate 2-methoxy-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.29
-
 ferricenium hexafluorophosphate substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 8.0 Phanerodontia chrysosporium
0.31
-
 2-chloro-1,4-benzoquinone substrate 2-chloro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.33
-
 ferricenium hexafluorophosphate substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.51
-
 2-methyl-1,4-benzoquinone substrate 2-methyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.64
-
 tetrafluoro-1,4-benzoquinone substrate tetrafluoro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.84
-
 D-glucose substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.22
-
 O2 substrate O2 (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.59
-
 2,6-dimethyl-1,4-benzoquinone substrate 2,6-dimethyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
2.94
-
 D-galactose substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
3.43
-
 ferricyanide substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
20.9
-
 D-xylose substrate D-xylose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
23.5
-
 L-sorbose substrate L-sorbose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
103
-
 D-fructose substrate D-fructose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 one subunit of recombinant expressed protein Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q6QWR1
-
-

Purification (Commentary)

Purification (Comment) Organism
homogenized in a French press, one-step immobilized metal affinity chromatography, concentrated in 50mM potassium phosphate buffer Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
2,6-dichloroindophenol + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
2,6-dimethyl-1,4-benzoquinone + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
2-chloro-1,4-benzoquinone + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
2-methoxy-1,4-benzoquinone + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
D-fructose + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
D-galactose + 1,4-benzoquinone
-
 Phanerodontia chrysosporium 2-dehydro-D-galactose + 1,4-hydroquinone
-
 ?
D-galactose + O2
-
 Phanerodontia chrysosporium 2-dehydro-D-galactose + H2O2
-
 ?
D-glucose + 1,4-benzoquinone
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + 1,4-hydroquinone
-
 ?
D-glucose + 2-methyl-1,4-benzoquinone
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + 2-methylhydroquinone
-
 r
D-glucose + ferricyanide
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + ferrocyanide
-
 r
D-glucose + O2
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + H2O2
-
 ?
D-xylose + O2
-
 Phanerodontia chrysosporium D-xylosone + H2O2
-
 ?
ferricenium hexafluorophosphate + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
L-sorbose + O2
-
 Phanerodontia chrysosporium 5-dehydro-D-fructose + H2O2
-
 ?
tetrafluoro-1,4-benzoquinone + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?

Subunits

Subunits Comment Organism
homotetramer suggested by observed molecular weight Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
P2Ox
-
 Phanerodontia chrysosporium
pyranose 2-Oxidase
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 2-methyl-1,4-benzoquinone substrate 2-methyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.15
-
 2-chloro-1,4-benzoquinone substrate 2-chloro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
0.3
-
 ferricyanide substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.33
-
 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical substrate 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.88
-
 D-fructose substrate D-fructose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
4.87
-
 D-galactose substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
6.61
-
 tetrafluoro-1,4-benzoquinone substrate tetrafluoro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
8.69
-
 2,6-dimethyl-1,4-benzoquinone substrate 2,6-dimethyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
40.1
-
 2-methoxy-1,4-benzoquinone substrate 2-methoxy-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
44.9
-
 D-xylose substrate D-xylose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
58.8
-
 L-sorbose substrate L-sorbose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
83.1
-
 D-glucose substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
108
-
 2,6-dichloroindophenol substrate 2,6-dichloroindophenol (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
109
-
 O2 substrate O2 (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
228
-
 ferricenium hexafluorophosphate substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
400
-
 1,4-benzoquinone substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
477
-
 1,4-benzoquinone substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 4.5 Phanerodontia chrysosporium
549
-
 ferricenium hexafluorophosphate substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 8.0 Phanerodontia chrysosporium