Cloned (Comment) | Organism |
---|---|
expression of flavocytochrome b2 flavin-binding domain in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic parameters and 2H kinetic isotope effects for the isolated flavin domain and intact, wild-type flavocytoochrome b2 | Saccharomyces cerevisiae | |
0.01 | - |
ferricytochrome c | pH 7.5, 25°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.023 | - |
ferricytochrome c | pH 7.5, 25°C, recombinantly expressed flavocytochrome b2 flavin-binding domain | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, recombinantly expressed flavocytochrome b2 flavin-binding domain, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinantly expressed flavocytochrome b2 flavin-binding domain | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | kcat/KM is 24000 fold lower with the recombinantly expressed flavocytochrome b2 flavin-binding domain compared to wild-type enzyme | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, recombinantly expressed flavocytochrome b2 flavin-binding domain, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
flavocytochrome b2 | - |
Saccharomyces cerevisiae |
L-lactate:cytochrome c oxidoreductase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic parameters and 2H kinetic isotope effects for the isolated flavin domain and intact, wild-type flavocytoochrome b2 | Saccharomyces cerevisiae | |
0.02 | - |
ferricytochrome c | pH 7.5, 25°C, recombinantly expressed flavocytochrome b2 flavin-binding domain | Saccharomyces cerevisiae | |
207 | - |
ferricytochrome c | pH 7.5, 25°C, wild-type enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | the C-terminal domain of the enzyme contains FMN | Saccharomyces cerevisiae | |
heme | the N-terminal domain of the enzyme contains protohaem IX | Saccharomyces cerevisiae |