Cloned (Comment) | Organism |
---|---|
in Escherichia coli MM294 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
Y254F | only traces of activity | Saccharomyces cerevisiae |
Y254L | turnover reduced to less than 10% | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-lactate | - |
Saccharomyces cerevisiae | |
L-Mandelate | competitive | Saccharomyces cerevisiae | |
oxalate | - |
Saccharomyces cerevisiae | |
pyruvate | competitive at low concentrations, non-competitve at high concentrations | Saccharomyces cerevisiae | |
sulfite | competitive | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial intermembrane space | - |
Saccharomyces cerevisiae | 5758 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to enzyme | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | can feed electrons to respiratory chain at the level of cytochrome c | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | can feed electrons to respiratory chain at the level of cytochrome c | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
additional information | ferricyanide used as electron acceptor | Saccharomyces cerevisiae | ? | - |
? | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | ferricyanide used as electron acceptor | Saccharomyces cerevisiae | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae |