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Literature summary for 1.1.1.B60 extracted from

  • Kim, T.S.; Patel, S.K.; Selvaraj, C.; Jung, W.S.; Pan, C.H.; Kang, Y.C.; Lee, J.K.
    A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization (2016), Sci. Rep., 6, 33438.
    View publication on PubMed
Search for more literature for 1.1.1.B60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)-CodonPlus RIL cells Gluconobacter oxydans

General Stability

General Stability Organism
stability of the enzyme significantly improves (up to 13.6fold) after cross-linking of immobilized enzyme on silica nanoparticles and retains 62.8% residual activity after 10 cycles of reuse Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
D-sorbitol inhibitory at more than 10% (w/v) Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
38.9
-
 D-sorbitol at pH 10.0 and 25°C Gluconobacter oxydans

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Gluconobacter oxydans
Ca2+ activates Gluconobacter oxydans
Cu2+ activates Gluconobacter oxydans
K+ activates Gluconobacter oxydans
additional information Mg2+, Co2+, and Mn2+ ions show no stimulatory influence on enzyme activity Gluconobacter oxydans
Zn2+ activates Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 2 * 54000, SDS-PAGE Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans G624
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinitol + NADP+
-
 Gluconobacter oxydans ?
-
 ?
D-arabinitol + NADP+
-
 Gluconobacter oxydans G624 ?
-
 ?
D-sorbitol + NADP+ best substrate Gluconobacter oxydans L-sorbose + NADPH + H+
-
 ?
D-sorbitol + NADP+ best substrate Gluconobacter oxydans G624 L-sorbose + NADPH + H+
-
 ?
mannitol + NADP+
-
 Gluconobacter oxydans ?
-
 ?
mannitol + NADP+
-
 Gluconobacter oxydans G624 ?
-
 ?
additional information no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol Gluconobacter oxydans ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 54000, SDS-PAGE Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
D-sorbitol dehydrogenase (NADP+)
-
 Gluconobacter oxydans
D-sorbitol:NADP+ oxidoreductase (L-sorbose-forming)
-
 Gluconobacter oxydans
SLDH
-
 Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Gluconobacter oxydans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 75 more than 40% activity between 25 and 75°C Gluconobacter oxydans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3820
-
 D-sorbitol at pH 10.0 and 25°C Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADP+ the enzyme exhibits high preference for NADP+ (vs. 2.5% relative activity with NAD+) Gluconobacter oxydans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
98.1
-
 D-sorbitol at pH 10.0 and 25°C Gluconobacter oxydans