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show all sequences of 1.1.1.B4

I86A/C295A mutant secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus has broadened substrate specificity for aryl ketones

Nealon, C.M.; Welsh, T.P.; Kim, C.S.; Phillips, R.S.; Arch. Biochem. Biophys. 606, 151-156 (2016)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
C295A
the mutant shows an increased size of the alkyl group which can bind in the substrate small pocket by one carbon atom compared to the wild-type enzyme
Thermoanaerobacter ethanolicus
I86A
the mutant shows altered substrate specificity and expands substrate specificity to include acetophenone, which is a very poor substrate for wild-type SADH, but also reverses the usual preferred stereochemistry to produce the anti-prelog R-product. I86A SADH exhibits limited reactivity with substituted acetophenones, fluorine being the only tolerable substituent
Thermoanaerobacter ethanolicus
I86A/C295A
the mutant enzyme shows broadened substrate specificity for aryl ketones and broadened substrate specificity for meta-substituted, but not para-substituted, acetophenones compared to the wild-type enzyme. The increase of the substrate specificity of I86A/C295A SADH is accompanied by a decrease in the kcat/Km values of acetophenones, possibly due to the substrates fitting loosely inside the more open active site
Thermoanaerobacter ethanolicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Dichloromethane
-
Thermoanaerobacter ethanolicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Thermoanaerobacter ethanolicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
required
Thermoanaerobacter ethanolicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermoanaerobacter ethanolicus
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme by a procedure including RedA-agarose affinity chromatography to electrophoretical homogeneity
Thermoanaerobacter ethanolicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-(2-pyridyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(2-pyridyl)ethanol + NADP+
-
-
-
r
1-(2-thienyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(2-thienyl)ethanol + NADP+
-
-
-
r
1-(3-pyridyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3-pyridyl)ethanol + NADP+
-
-
-
r
1-(3-thienyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3-thienyl)ethanol + NADP+
-
-
-
r
1-(4-pyridyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(4-pyridyl)ethanol + NADP+
-
-
-
r
1-phenylpropanone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-phenylpropanol + NADP+
-
-
-
r
2',4'-difluoro-acetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(2',4'-difluoro)-phenylethanol + NADP+
-
-
-
r
2'-methylacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(2'-methyl)phenylethanol + NADP+
-
-
-
r
3'-bromo-acetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-bromo-)phenylethanol + NADP+
-
-
-
r
3'-chloroacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-chlorophenyl)ethanol + NADP+
-
-
-
r
3'-iodoacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-iodophenyl)ethanol + NADP+
-
-
-
r
3'-methoxyacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-methoxyphenyl)ethanol + NADP+
-
-
-
r
3'-methylacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-methylphenyl)ethanol + NADP+
-
-
-
r
3'-trifluoromethylacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-[(3'-trifluoromethyl)phenyl]ethanol + NADP+
-
-
-
r
acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
2-propanol + NADP+
-
-
-
r
acetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-phenylethanol + NADP+
-
-
-
r
additional information
the enzyme mutant I86A/C295A SADH gives high conversions and very high enantiomeric excess of the anti-Prelog R-alcohols from the tested substrates, it shows broad substrate specificity for meta-substituted, but not para-substituted, acetophenones and aryl ketones
741784
Thermoanaerobacter ethanolicus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 37700, SDS-PAGE
Thermoanaerobacter ethanolicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Thermoanaerobacter ethanolicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.04
-
3'-trifluoro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.08
-
3'-methoxy-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.18
-
2'-methylacetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.47
-
1-(3-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.61
-
3'-iodo-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.66
-
3'-chloro-acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.01
-
acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.5
-
1-(2-thienyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.7
-
1-(2-thienyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.7
-
1-(4-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
2
-
3'-methylacetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
2.2
-
2',4'-difluoro-acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
2.31
-
1-(2-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
2.9
-
2',4'-difluoro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
6.5
-
1-phenylpropanone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
6.5
-
3'-bromo-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
6.9
-
1-(2-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
8.2
-
acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
12.3
-
3'-chloro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
13.1
-
1-(4-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
14.9
-
1-(3-thienyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
15.8
-
acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
23.3
-
1-phenylpropanone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
35
-
acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Thermoanaerobacter ethanolicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Thermoanaerobacter ethanolicus
NADPH
-
Thermoanaerobacter ethanolicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Thermoanaerobacter ethanolicus
NADPH
-
Thermoanaerobacter ethanolicus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C295A
the mutant shows an increased size of the alkyl group which can bind in the substrate small pocket by one carbon atom compared to the wild-type enzyme
Thermoanaerobacter ethanolicus
I86A
the mutant shows altered substrate specificity and expands substrate specificity to include acetophenone, which is a very poor substrate for wild-type SADH, but also reverses the usual preferred stereochemistry to produce the anti-prelog R-product. I86A SADH exhibits limited reactivity with substituted acetophenones, fluorine being the only tolerable substituent
Thermoanaerobacter ethanolicus
I86A/C295A
the mutant enzyme shows broadened substrate specificity for aryl ketones and broadened substrate specificity for meta-substituted, but not para-substituted, acetophenones compared to the wild-type enzyme. The increase of the substrate specificity of I86A/C295A SADH is accompanied by a decrease in the kcat/Km values of acetophenones, possibly due to the substrates fitting loosely inside the more open active site
Thermoanaerobacter ethanolicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Dichloromethane
-
Thermoanaerobacter ethanolicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Thermoanaerobacter ethanolicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
required
Thermoanaerobacter ethanolicus
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme by a procedure including RedA-agarose affinity chromatography to electrophoretical homogeneity
Thermoanaerobacter ethanolicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-(2-pyridyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(2-pyridyl)ethanol + NADP+
-
-
-
r
1-(2-thienyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(2-thienyl)ethanol + NADP+
-
-
-
r
1-(3-pyridyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3-pyridyl)ethanol + NADP+
-
-
-
r
1-(3-thienyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3-thienyl)ethanol + NADP+
-
-
-
r
1-(4-pyridyl)acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(4-pyridyl)ethanol + NADP+
-
-
-
r
1-phenylpropanone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-phenylpropanol + NADP+
-
-
-
r
2',4'-difluoro-acetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(2',4'-difluoro)-phenylethanol + NADP+
-
-
-
r
2'-methylacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(2'-methyl)phenylethanol + NADP+
-
-
-
r
3'-bromo-acetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-bromo-)phenylethanol + NADP+
-
-
-
r
3'-chloroacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-chlorophenyl)ethanol + NADP+
-
-
-
r
3'-iodoacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-iodophenyl)ethanol + NADP+
-
-
-
r
3'-methoxyacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-methoxyphenyl)ethanol + NADP+
-
-
-
r
3'-methylacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-(3'-methylphenyl)ethanol + NADP+
-
-
-
r
3'-trifluoromethylacetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-[(3'-trifluoromethyl)phenyl]ethanol + NADP+
-
-
-
r
acetone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
2-propanol + NADP+
-
-
-
r
acetophenone + NADPH + H+
-
741784
Thermoanaerobacter ethanolicus
1-phenylethanol + NADP+
-
-
-
r
additional information
the enzyme mutant I86A/C295A SADH gives high conversions and very high enantiomeric excess of the anti-Prelog R-alcohols from the tested substrates, it shows broad substrate specificity for meta-substituted, but not para-substituted, acetophenones and aryl ketones
741784
Thermoanaerobacter ethanolicus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37700, SDS-PAGE
Thermoanaerobacter ethanolicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Thermoanaerobacter ethanolicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.04
-
3'-trifluoro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.08
-
3'-methoxy-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.18
-
2'-methylacetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.47
-
1-(3-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.61
-
3'-iodo-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.66
-
3'-chloro-acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.01
-
acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.5
-
1-(2-thienyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.7
-
1-(2-thienyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.7
-
1-(4-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
2
-
3'-methylacetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
2.2
-
2',4'-difluoro-acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
2.31
-
1-(2-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
2.9
-
2',4'-difluoro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
6.5
-
1-phenylpropanone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
6.5
-
3'-bromo-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
6.9
-
1-(2-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
8.2
-
acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
12.3
-
3'-chloro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
13.1
-
1-(4-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
14.9
-
1-(3-thienyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
15.8
-
acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
23.3
-
1-phenylpropanone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
35
-
acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Thermoanaerobacter ethanolicus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.018
-
2'-methylacetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.025
-
3'-trifluoro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.16
-
3'-iodo-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.18
-
3'-methylacetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.2
-
1-(3-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.21
-
3'-methoxy-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.23
-
2',4'-difluoro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.26
-
1-(2-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.26
-
1-(2-thienyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.35
-
1-(2-thienyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.41
-
3'-chloro-acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.51
-
1-(2-thienyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.62
-
2',4'-difluoro-acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.63
-
1-(3-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.65
-
3'-bromo-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.05
-
1-(4-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.08
-
acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.3
-
3'-chloro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.33
-
acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.5
-
1-phenylpropanone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.6
-
1-(2-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.9
-
acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
2.4
-
1-(4-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
4.3
-
1-(3-thienyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
8.1
-
1-phenylpropanone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
14.9
-
acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.018
-
2'-methylacetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.025
-
3'-trifluoro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.16
-
3'-iodo-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.18
-
3'-methylacetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.2
-
1-(3-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.21
-
3'-methoxy-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.23
-
2',4'-difluoro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.26
-
1-(2-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.26
-
1-(2-thienyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.35
-
1-(2-thienyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.41
-
3'-chloro-acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.51
-
1-(2-thienyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.62
-
2',4'-difluoro-acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
0.63
-
1-(3-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
0.65
-
3'-bromo-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.05
-
1-(4-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.08
-
acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.3
-
3'-chloro-acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.33
-
acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.5
-
1-phenylpropanone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
1.6
-
1-(2-pyridyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
1.9
-
acetophenone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
2.4
-
1-(4-pyridyl)acetone
pH 6.5, 50°C, wild-type enzyme
Thermoanaerobacter ethanolicus
4.3
-
1-(3-thienyl)acetone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
8.1
-
1-phenylpropanone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
14.9
-
acetophenone
pH 6.5, 50°C, mutant I86A
Thermoanaerobacter ethanolicus
Other publictions for EC 1.1.1.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741784
Nealon
I86A/C295A mutant secondary a ...
Thermoanaerobacter ethanolicus
Arch. Biochem. Biophys.
606
151-156
2016
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3
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1
1
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1
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2
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1
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17
1
1
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24
1
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2
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2
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3
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1
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1
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1
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1
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17
1
1
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24
1
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26
26
742305
Peng
-
Continuous preparation of (R) ...
Morganella morganii, Morganella morganii CMCC(B)49208
Chin. J. Proc. Engin.
16
286-291
2016
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1
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2
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2
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1
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4
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1
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1
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740394
Chen
Cloning, expression, and chara ...
Gluconobacter oxydans, Gluconobacter oxydans DSM 2343
Enzyme Microb. Technol.
70
18-27
2015
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-
-
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2
3
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3
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2
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13
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1
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1
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2
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2
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3
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3
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13
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1
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2
1
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2
2
739942
Wang
Cloning, expression, and direc ...
Leifsonia xyli, Leifsonia xyli HS0904
Appl. Microbiol. Biotechnol.
98
8591-8601
2014
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1
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2
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6
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2
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2
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6
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1
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6
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2
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6
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6
6
743703
He
-
Purification and characteriza ...
Kocuria rhizophila
Process Biochem.
49
1107-1112
2014
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1
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9
5
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5
1
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1
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1
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1
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19
1
1
1
3
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1
1
1
3
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1
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3
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9
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5
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5
1
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1
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1
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19
1
1
1
3
-
1
1
1
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-
721911
Zhou
Biocatalytic characterization ...
Carboxydothermus hydrogenoformans, Carboxydothermus hydrogenoformans DSM 6008
Biotechnol. Lett.
35
359-365
2013
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1
1
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1
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1
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4
2
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15
1
1
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1
1
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2
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1
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1
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4
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15
1
1
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1
1
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-
739805
Aggarwal
Expression, purification, crys ...
Candida parapsilosis, Candida parapsilosis ATCC 7330
Acta Crystallogr. Sect. F
69
313-315
2013
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1
1
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2
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2
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723841
Protsko
Crystallization and preliminar ...
Thermoanaerobacter ethanolicus
Acta Crystallogr. Sect. F
66
831-833
2010
-
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1
1
1
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1
1
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3
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1
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1
1
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2
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1
2
1
1
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1
1
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1
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1
1
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-
-
-
-
-
1
1
-
-
-
725726
Li
-
Deracemization of aryl seconda ...
Microbacterium oxydans, Microbacterium oxydans ECU2010
J. Mol. Catal. B
64
48-52
2010
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1
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2
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4
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697267
Pal
Activity of yeast alcohol dehy ...
Saccharomyces cerevisiae, Saccharomyces pastorianus, Saccharomyces pastorianus Y379-50
Chem. Biol. Interact.
178
16-23
2009
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697839
Pennacchio
Role of Tryptophan 95 in subst ...
Saccharolobus solfataricus
Extremophiles
13
751-761
2009
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1
1
1
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4
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711860
Machielsen
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Cofactor engineering of Lactob ...
Lactobacillus brevis
Eng. Life Sci.
9
38-44
2009
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2
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4
4
684583
Pennacchio
Purification and characterizat ...
Thermus thermophilus
Appl. Environ. Microbiol.
74
3949-3958
2008
1
1
1
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7
5
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4
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1
1
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1
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7
1
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1
4
4
2
2
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1
1
1
3
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7
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7
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4
4
2
2
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688747
Nie
A novel NADH-dependent carbony ...
Candida parapsilosis
Lett. Appl. Microbiol.
44
555-562
2007
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10
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687864
Isobe
Thermostable NAD+-dependent (R ...
Burkholderia sp., Burkholderia sp. AIU 652
J. Biosci. Bioeng.
96
387-393
2003
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15
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688571
Bradshaw
-
A Pseudomonas sp. alcohol dehy ...
Pseudomonas sp., Pseudomonas sp. PED
J. Org. Chem.
57
1526-1532
1992
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