Application | Comment | Organism |
---|---|---|
molecular biology | a high thermostability of PsXDH is obtained by subsequent site-directed mutagenesis of the structural zinc-binding loop. The best mutant in this study (C4/F98R/E101F) shows a 10.8 °C higher thermal transition temperature and 20.8 °C higher half denaturation temperature (T1/2) compared with wild-type | Scheffersomyces stipitis |
Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Scheffersomyces stipitis |
Protein Variants | Comment | Organism |
---|---|---|
S96C/S99C/Y102C | specific activity (U/min): 1440, half denaturation temperature T1/2 (°C): 46.1, thermal transition temperature Tcd (°C): 47.5 | Scheffersomyces stipitis |
S96C/S99C/Y102C/E101F | specific activity (U/min): 1550, half denaturation temperature T1/2 (°C): 50.9, thermal transition temperature Tcd (°C): 50.5 | Scheffersomyces stipitis |
S96C/S99C/Y102C/F98R | specific activity (U/min): 1510, half denaturation temperature T1/2 (°C): 53.1, thermal transition temperature Tcd (°C): 51.7 | Scheffersomyces stipitis |
S96C/S99C/Y102C/F98R/E101F | specific activity (U/min): 1620, half denaturation temperature T1/2 (°C): 56.0, thermal transition temperature Tcd (°C): 53.8 | Scheffersomyces stipitis |
S96C/S99C/Y102C/H112D | specific activity (U/min): 1360, half denaturation temperature T1/2 (°C): 44.0, thermal transition temperature Tcd (°C): 47.0 | Scheffersomyces stipitis |
S96C/S99C/Y102C/P95S | specific activity (U/min): 1220, half denaturation temperature T1/2 (°C): 37.4, thermal transition temperature Tcd (°C): 43.5 | Scheffersomyces stipitis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Scheffersomyces stipitis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Scheffersomyces stipitis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity (U/min): 1110 (wild-type), 1440 (mutant S96C/S99C/Y102C), 1220 (mutant S96C/S99C/Y102C/P95S), 1510 (mutant S96C/S99C/Y102C/F98R), 1550 (mutant S96C/S99C/Y102C/E101F), 1360 (mutant S96C/S99C/Y102C/H112D), 1620 (mutant S96C/S99C/Y102C/F98R/E101F) | Scheffersomyces stipitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | - |
Scheffersomyces stipitis | D-xylulose + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PsXDH | - |
Scheffersomyces stipitis |
xylitol dehydrogenase | - |
Scheffersomyces stipitis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Scheffersomyces stipitis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type: half denaturation temperature T1/2 (°C): 35.2, thermal transition temperature Tcd (°C): 43.0 | Scheffersomyces stipitis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Scheffersomyces stipitis |