Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no significant content of Mg2+ | Candida sp. HA 167 | |
Zn2+ | 0.9 molecules per subunit | Candida sp. HA 167 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, SDS-PAGE | Candida sp. HA 167 |
160000 | - |
gel filtration | Candida sp. HA 167 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida sp. HA 167 | - |
expressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Candida sp. HA 167 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | - |
Candida sp. HA 167 | D-sorbitol + NAD+ | - |
? | |
D-sorbitol + NAD+ | - |
Candida sp. HA 167 | D-fructose + NADH + H+ | - |
r | |
D-xylitol + NAD+ | - |
Candida sp. HA 167 | D-xylulose + NADH + H+ | - |
r | |
D-xylulose + NADH + H+ | specific for transferring the 4-pro-R hydrogen of NADH | Candida sp. HA 167 | D-xylitol + NAD+ | - |
r | |
additional information | specific for transferring the 4-pro-R hydrogen of NADH | Candida sp. HA 167 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 38000, SDS-PAGE | Candida sp. HA 167 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
114 | - |
D-sorbitol | 25°C, pH 7.5, Tris-HCl | Candida sp. HA 167 | |
143 | - |
xylitol | 25°C, pH 7.5, Tris-HCl | Candida sp. HA 167 | |
170 | - |
xylitol | 25°C, pH 7.5, potassium phosphate | Candida sp. HA 167 | |
920 | - |
D-fructose | 25°C, pH 7.5, Tris-HCl | Candida sp. HA 167 | |
1500 | - |
D-xylulose | 25°C, pH 7.5, Tris-HCl | Candida sp. HA 167 | |
1800 | - |
D-xylulose | 25°C, pH 7.5, potassium phosphate | Candida sp. HA 167 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Candida sp. HA 167 | |
NADH | - |
Candida sp. HA 167 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Candida sp. HA 167 | isoelectric focusing | - |
6 |