General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification | Klebsiella aerogenes |
EDTA stabilizes during purification | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Klebsiella aerogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Klebsiella aerogenes | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation at high concentration | Klebsiella aerogenes | |
Mn2+ | requirement | Klebsiella aerogenes | |
additional information | no activation by Zn2+, Cu2+, Ca2+, Ni2+, Co2+, Fe2+ | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
2°C, 90% of maximal activity retained at pH 6.8 and 7.4 after 24 h | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol + NAD+ | - |
Klebsiella aerogenes | ? | - |
? | |
D-xylulose + NADH + H+ | - |
Klebsiella aerogenes | xylitol + NAD+ | - |
r | |
dihydroxyacetone + NADH | - |
Klebsiella aerogenes | glycerol + NAD+ | - |
? | |
erythritol + NAD+ | - |
Klebsiella aerogenes | L-erythrulose + NADH | - |
r | |
glycerol + NAD+ | - |
Klebsiella aerogenes | ? | - |
? | |
L-arabitol + NAD+ | - |
Klebsiella aerogenes | ? | - |
? | |
L-erythrulose + NADH | - |
Klebsiella aerogenes | erythritol + NAD+ | - |
r | |
L-xylulose + NADH | - |
Klebsiella aerogenes | L-xylitol + NAD+ | - |
? | |
additional information | no oxidation of threitol, xylitol, sorbitol, D-iditol | Klebsiella aerogenes | ? | - |
? | |
additional information | no reduction of D-fructose, L-sorbose and D-tagatose | Klebsiella aerogenes | ? | - |
? | |
additional information | specific for polyols of 5 or less carbon bearing cis-hydroxy groups in C2 and C3 | Klebsiella aerogenes | ? | - |
? | |
xylitol + NAD+ | - |
Klebsiella aerogenes | D-xylulose + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
reduction of erythrulose | Klebsiella aerogenes |
8.6 | - |
oxidation of erythritol | Klebsiella aerogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.4 | 90% of maximal activity retained at pH 6.8 and 7.4 at 2°C | Klebsiella aerogenes |
7 | - |
24 h stable at 2°C | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Klebsiella aerogenes | |
additional information | no activity with NADP+ | Klebsiella aerogenes | |
additional information | at high concentrations of xylitol 1.5% as effective as NAD+ | Klebsiella aerogenes | |
NAD+ | - |
Klebsiella aerogenes | |
NADH | - |
Klebsiella aerogenes |