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Literature summary for 1.1.1.87 extracted from
- Enzymatic formation of alpha-ketoadipic acid from homoisocitric acid (1965), J. Biol. Chem., 240, 4357-4361.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
3-carboxy-2-hydroxyadipate | - |
Saccharomyces cerevisiae |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation, 2fold activity at 5 mM | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-carboxy-2-hydroxyadipate + NAD+ | Saccharomyces cerevisiae | intermediate in lysine biosynthesis | 2-oxoadipate + CO2 + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, ammonium sulfate precipitate, partially purified, stable | Saccharomyces cerevisiae |
| 0°C, potassium phosphate 20 mM, pH 7.2, 4-5 hours | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-carboxy-2-hydroxyadipate + NAD+ | - |
Saccharomyces cerevisiae | 2-oxoadipate + CO2 + NADH | - |
? | |
| 3-carboxy-2-hydroxyadipate + NAD+ | intermediate in lysine biosynthesis | Saccharomyces cerevisiae | 2-oxoadipate + CO2 + NADH | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | activity drops sharply below pH 6.5 and above pH 8.5 | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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