Protein Variants | Comment | Organism |
---|---|---|
D236R/D289K/I290Y/A296V/G337Y | engineering of the enzyme by site-directed mutagenesis in the cofactor binding pocket to utilize NADP+ as cofactor instead of NAD+, specificity change of factor 20000, modified cofactor binding structure, the mutant shows the same specificity for NADP+ as the wild-type enzyme for NAD+, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the enzyme catalyzes a step in leucine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes a step in leucine biosynthesis | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMDH | - |
Escherichia coli |
isopropylmalate dehydrogenase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor binding structure, 100fold preference to NADP+ | Escherichia coli |