General Stability | Organism |
---|---|
in contrast to 3-isopropylmalate dehydrogenase from other sources, K+ and Na+ are not essential for stability of the Sulfolobus enzyme | Sulfolobus sp. |
K+ does not stabilize the enzyme | Sulfolobus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sulfolobus sp. | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | divalent cation required | Sulfolobus sp. | |
Cd2+ | divalent cation required, 93% of the activity with Mn2+ | Sulfolobus sp. | |
Cd2+ | required for activity, 0.2 M | Sulfolobus sp. | |
Co2+ | divalent cation required | Sulfolobus sp. | |
Co2+ | divalent cation required, 43% of the activity with Mn2+ | Sulfolobus sp. | |
Co2+ | required for activity, 0.2 M | Sulfolobus sp. | |
K+ | 1.5fold stimulation, optimal concentration: 0.2 M | Sulfolobus sp. | |
K+ | slight stimulation, but not essential. Optimal concentration 0.2 M | Sulfolobus sp. | |
K+ | about 1.5fold stimulation, optimal concentration around 0.2 M | Sulfolobus sp. | |
Mg2+ | divalent cation required | Sulfolobus sp. | |
Mg2+ | divalent cation required, 76% of the activity with Mn2+ | Sulfolobus sp. | |
Mg2+ | required for activity, 0.2 M | Sulfolobus sp. | |
Mn2+ | divalent cation required | Sulfolobus sp. | |
Mn2+ | divalent cation required, maximum activity in presence of Mn2+ | Sulfolobus sp. | |
Mn2+ | required for activity, 0.2 M | Sulfolobus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
3 * 36000, or 4 * 360000, SDS-PAGE | Sulfolobus sp. |
36000 | - |
3 * or 4 * 36000, SDS-PAGE | Sulfolobus sp. |
110000 | - |
gel filtration | Sulfolobus sp. |
360000 | - |
3 * 36000, or 4 * 360000, SDS-PAGE | Sulfolobus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus sp. | - |
- |
- |
Sulfolobus sp. 7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.4 | - |
pH 7.0, 55°C | Sulfolobus sp. |
19.4 | - |
55°C | Sulfolobus sp. |
Subunits | Comment | Organism |
---|---|---|
? | 3 * or 4 * 36000, SDS-PAGE | Sulfolobus sp. |
dimer | 3 * 36000, or 4 * 360000, SDS-PAGE | Sulfolobus sp. |
tetramer | or trimer, 4* 36000, SDS-PAGE | Sulfolobus sp. |
trimer | 3 * 36000, or 4 * 360000, SDS-PAGE | Sulfolobus sp. |
trimer | or tetramer, 3* 36000, SDS-PAGE | Sulfolobus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Sulfolobus sp. |
75 | - |
- |
Sulfolobus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
97 | - |
melting temperature | Sulfolobus sp. |
97 | - |
Tm-value | Sulfolobus sp. |
97 | - |
pH 7.0, 50% inactivation | Sulfolobus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Sulfolobus sp. |
7.5 | 8.5 | - |
Sulfolobus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sulfolobus sp. | |
NADH | - |
Sulfolobus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sulfolobus sp. | isoelectric focusing | - |
6.9 |