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Literature summary for 1.1.1.81 extracted from

  • Sugimoto, E.; Kitagawa, Y.; Nakanishi, K.; Chiba, H.
    Purification and properties of beef liver D-glycerate dehydrogenase (1972), J. Biochem., 72, 1307-1315.
    View publication on PubMed
Search for more literature for 1.1.1.81

General Stability

General Stability Organism
dithiothreitol stabilizes Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2,3-diphospho-D-glycerate 1 mM, 83% and 91% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively Bos taurus
2-phospho-DL-glycerate 1 mM, 93% and 81% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively Bos taurus
3-phospho-D-glycerate 1 mM, 53% and 65% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively Bos taurus
Ag+ 0.1 mM, complete inhibition Bos taurus
alpha-D-fructose 1,6-diphosphate 0.1 mM, 28% and 74% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively Bos taurus
ATP 1 mM, 62% and 64% inhibition of liver and spinal cord enzyme respectively, 73% and 89% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively Bos taurus
Hg2+ 0.1 mM, complete inhibition Bos taurus
p-chloromercuribenzoate 0.1 mM, complete inhibition Bos taurus
pyruvate 1 mM, 73% and 89% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 Hydroxypyruvate
-
 Bos taurus
0.037
-
 NADP+
-
 Bos taurus
0.077
-
 NAD+
-
 Bos taurus
1.4
-
 DL-glycerate
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 sedimentation velocity analysis Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxypyruvate + NAD(P)H Bos taurus
-
 D-glycerate + NAD(P)+ + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.67
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-glycerate + NAD+
-
 Bos taurus 3-hydroxypyruvate + NADH + H+
-
 r
hydroxypyruvate + NAD(P)H
-
 Bos taurus D-glycerate + NAD(P)+ + H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 hydroxypyruvate reduction Bos taurus
9.3
-
 D-glycerate oxidation Bos taurus