Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibition | Bacillus licheniformis | |
Al3+ | strong inhibition | Bacillus licheniformis | |
Cu2+ | strong inhibition | Bacillus licheniformis | |
Fe3+ | strong inhibition | Bacillus licheniformis | |
K+ | slight inhibition | Bacillus licheniformis | |
additional information | not inhibited by EDTA | Bacillus licheniformis | |
Zn2+ | slight inhibition | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
(2S)-acetoin | at pH 10.0 and 30°C | Bacillus licheniformis | |
7.25 | - |
(R,R)-butane-2,3-diol | at pH 10.0 and 30°C | Bacillus licheniformis | |
72.4 | - |
diacetyl | at pH 5.0 and 30°C | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight activation | Bacillus licheniformis | |
Mn2+ | slight activation | Bacillus licheniformis | |
additional information | not influenced by Ni2+, Ba2+, Li+, Ca2+, and Na+ | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, SDS-PAGE | Bacillus licheniformis |
125000 | - |
gel filtration | Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-butane-2,3-diol + NAD+ | Bacillus licheniformis | 100% activity | (2S)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-acetoin + NADH + H+ | 97% activity compared to diacetyl | Bacillus licheniformis | (2S,3S)-butane-2,3-diol + NAD+ | - |
r | |
(2S,3S)-butane-2,3-diol + NAD+ | 100% activity | Bacillus licheniformis | (2S)-acetoin + NADH + H+ | - |
r | |
(R,R)-butane-2,3-diol + NAD+ | - |
Bacillus licheniformis | ? | - |
? | |
1,2-propanediol + NAD+ | 0.57% activity compared to (2S,3S)-butane-2,3-diol | Bacillus licheniformis | ? | - |
? | |
2,3-hexanedione + NADH + H+ | 66% activity compared to diacetyl | Bacillus licheniformis | ? | - |
? | |
2,3-pentanedione + NADH + H+ | 69% activity compared to diacetyl | Bacillus licheniformis | ? | - |
? | |
3,4-hexanedione + NADH + H+ | 10% activity compared to diacetyl | Bacillus licheniformis | ? | - |
? | |
diacetyl + NADH + H+ | 100% activity | Bacillus licheniformis | (2S)-acetoin + NAD+ | - |
? | |
additional information | the enzyme shows no activity toward racemic acetoin in the presence of NAD+ as well as no activity with NADPH, 1,4-butanediol, 2,5-hexanedione, 2,4-pentanedione, 2-butanone, methanol, mannitol, and glycerol | Bacillus licheniformis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 30000, SDS-PAGE | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Bacillus licheniformis |
budC | - |
Bacillus licheniformis |
meso-2,3-butanediol dehydrogenase | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
at 50°C, the enzymatic activity drops rapidly to half of its initial activity within 40 min, then further decreases to 20% after 330 min | Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
202 | - |
(2S)-acetoin | at pH 10.0 and 30°C | Bacillus licheniformis | |
591 | - |
(R,R)-butane-2,3-diol | at pH 10.0 and 30°C | Bacillus licheniformis | |
1222 | - |
diacetyl | at pH 5.0 and 30°C | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
for diacetyl reduction | Bacillus licheniformis |
10 | - |
for 2,3-butanediol oxidation | Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | the enzyme shows a relatively high activity over a wide pH range from 5.0 to 8.0 for racemic acetoin reduction and more than 60% of activity is retained between pH 4.5 and 10.0 | Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus licheniformis | |
NADH | - |
Bacillus licheniformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.9 | - |
diacetyl | at pH 5.0 and 30°C | Bacillus licheniformis | |
81.5 | - |
(R,R)-butane-2,3-diol | at pH 10.0 and 30°C | Bacillus licheniformis | |
432 | - |
(2S)-acetoin | at pH 10.0 and 30°C | Bacillus licheniformis |