Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | about 65% inhibition at 5 mM | Camelus dromedarius | |
Ca2+ | about 90% residual activity at 2 mM | Camelus dromedarius | |
Cu2+ | about 15% residual activity at 5 mM | Camelus dromedarius | |
D-glucose 6-phosphate | about 48% inhibition at 5 mM | Camelus dromedarius | |
dithiothreitol | about 40% inhibition at 5 mM | Camelus dromedarius | |
Fe2+ | about 30% residual activity at 5 mM | Camelus dromedarius | |
additional information | less than 8% inhibition with 1,10-phenanthroline, ATP, EDTA, and iodoacetamide. Not inhibited by NAD+ | Camelus dromedarius | |
NADH | about 38% inhibition at 5 mM | Camelus dromedarius | |
NADPH | about 90% inhibition at 0.2 mM | Camelus dromedarius | |
Ni2+ | about 95% residual activity at 2 mM | Camelus dromedarius | |
phenylmethylsulfonyl fluoride | about 48% inhibition at 5 mM | Camelus dromedarius | |
Zn2+ | about 50% residual activity at 5 mM | Camelus dromedarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
NADP+ | at pH 8.0 temperature not specified in the publication | Camelus dromedarius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 110% activity at 5 mM | Camelus dromedarius | |
Mg2+ | about 135% activity at 5 mM | Camelus dromedarius | |
Mn2+ | about 115% activity at 5 mM | Camelus dromedarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
3 * 64000, SDS-PAGE | Camelus dromedarius |
194000 | - |
gel filtration | Camelus dromedarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Camelus dromedarius | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelus dromedarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE cellulose column chromatography, Sephacryl S-300 gel filtration, and 2',5' ADP Sepharose 4B affinity chromatography | Camelus dromedarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Camelus dromedarius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0286 | - |
crude extract, at pH 8.0 temperature not specified in the publication | Camelus dromedarius |
1.804 | - |
after 63.09fold purification, at pH 8.0 temperature not specified in the publication | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Camelus dromedarius | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 64000, SDS-PAGE | Camelus dromedarius |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucose-6-phosphate; NADP oxidoreductase | - |
Camelus dromedarius |
G6PD | - |
Camelus dromedarius |
glucose-6-phosphate dehydrogenase | - |
Camelus dromedarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Camelus dromedarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | more than 50% activity between pH 6.5 and 8.5 | Camelus dromedarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Camelus dromedarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Camelus dromedarius | isoelectric focusing | 6.8 | 6.6 |