Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
E96A | mutant enzyme has higher stability at 60°C and 90% remaining activity compared to the wild type enzyme | Priestia megaterium |
E96G | mutant enzyme has higher stability at 60°C and 47% remaining activity compared to the wild type enzyme | Priestia megaterium |
E96K/D108N/P194Q/E210K | mutant enzyme has higher stability at 60°C and 97% remaining activity compared to the wild type enzyme | Priestia megaterium |
E96K/V112A/E133K/Y217H | mutant enzyme has higher stability at 60°C and 85% remaining activity compared to the wild type enzyme | Priestia megaterium |
E96K/V183I | mutant enzyme has higher stability at 60°C and 72% remaining activity compared to the wild type enzyme | Priestia megaterium |
Q252L | mutant enzyme has higher stability at 60°C and 56% remaining activity compared to the wild type enzyme | Priestia megaterium |
Q252L/A258G | mutant enzyme has higher stability at 60°C and 61% remaining activity compared to the wild type enzyme | Priestia megaterium |
Y253C | mutant enzyme has higher stability at 60°C and 1% remaining activity compared to the wild type enzyme | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28090 | - |
subunit, calculated from amino acid sequence | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | P40288 | - |
- |
Priestia megaterium IWG3 | P40288 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephadex A-50 gel filtration | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + NAD+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose + NAD+ | - |
Priestia megaterium IWG3 | D-glucono-1,5-lactone + NADH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium |