Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of the catalytic alpha-subunit in Escherichia coli as active water-soluble protein | Burkholderia cepacia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by EDTA and thiol reagents such as fluorescein 5-maleimide | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
beta-D-glucose | pH 7.0, 70°C | Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme does not contain weakly associated metal ions | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, recombinant catalytic alpha-subunit, SDS-PAGE | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | Q8GQE7 | FERM BP-7306 strain SM-4 | - |
Purification (Comment) | Organism |
---|---|
recombinant catalytic alpha-subunit from Escherichia coli | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2000 | - |
above, purified recombinant catalytic alpha-subunit, 40°C | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + NAD(P)+ | - |
Burkholderia cepacia | D-glucono-1,5-lactone + NAD(P)H + H+ | - |
ir | |
D-galactose + NAD(P)+ | - |
Burkholderia cepacia | D-galactono-1,5-lactone + NAD(P)H | - |
? | |
additional information | Cys residues are not involved in the catalytic site | Burkholderia cepacia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant catalytic alpha-subunit, SDS-PAGE | Burkholderia cepacia |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Burkholderia cepacia |
glucose dehydrogenase | - |
Burkholderia cepacia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Burkholderia cepacia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermostable enzyme | Burkholderia cepacia |
60 | - |
30 min, stable | Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the catalytic alpha-subunit contains a FAD binding motif at the N-terminal region | Burkholderia cepacia | |
NAD+ | - |
Burkholderia cepacia | |
NADH | - |
Burkholderia cepacia | |
NADP+ | - |
Burkholderia cepacia | |
NADPH | - |
Burkholderia cepacia |