Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M sodium citrate, pH 5.5, 16% (w/v) PEG 4000, 20% (v/v) isopropanol | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C245S | the activities of the mutant in the presence of Cd2+ are decreased as that of wild type enzyme | Mus musculus |
C379S | the activity of the mutant in the absence of Cd2+ is similar to that of the wild type enzyme, but the decrease of activity in the presence of Cd2+ is much reduced | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | above 0.1 mM, the enzyme loses activity, and at 2 mM Cd2+ most of the activity has disappeared. Chelation of Cd2+ by dithiothreitol cannot recover the lost enzyme activity. Inactivation of the enzyme by Cd2+ is less effective when the enzyme is activated with Cd2+ than Mg2+, More than 50% of the activity of the enzyme activated with 0.05 mM Cd2+ remains in the presence of 1 mM GSH | Mus musculus | |
Mn2+ | the specific activity of purified enzyme is increased by Mn2+ in the micromolar range | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | the enzyme activity is increased at a Cd2+ concentration below 0.1 mM | Mus musculus | |
Mg2+ | the maximum activity with Mg2+ is slightly lower than that with Cd2+ | Mus musculus | |
additional information | Ca2+ does not activate Mg2+ | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Mus musculus | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | O88844 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Mus musculus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Mus musculus |
NADP+-dependent isocitrate dehydrogenase | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mus musculus |