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Literature summary for 1.1.1.40 extracted from

  • Huergo, L.F.; Araujo, G.A.T.; Santos, A.S.R.; Gerhardt, E.C.M.; Pedrosa, F.O.; Souza, E.M.; Forchhammer, K.
    The NADP-dependent malic enzyme MaeB is a central metabolic hub controlled by the acetyl-CoA to CoASH ratio (2020), Biochim. Biophys.Acta, 1868, 140462 .
    View publication on PubMed
Search for more literature for 1.1.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(lambdaDE3) cells Azospirillum brasilense

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate slight inhibition at 2 mM Azospirillum brasilense
acetyl phosphate slight inhibition at 2 mM Azospirillum brasilense
acetyl-CoA enzyme inhibition by acetyl-CoA is relieved by increasing CoASH concentrations Azospirillum brasilense
ADP slight inhibition at 2 mM Azospirillum brasilense
ATP slight inhibition at 2 mM Azospirillum brasilense
cAMP slight inhibition at 1 mM Azospirillum brasilense
CoA slight inhibition at 1 mM Azospirillum brasilense
glutamine slight inhibition at 5 mM Azospirillum brasilense
phosphoenolpyruvate slight inhibition at 1 mM Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.157
-
 NADP+ at pH 8.0 and 30°C Azospirillum brasilense
2.9
-
 (S)-malate in the presence of 1 mM NADP+, at pH 8.0 and 30°C Azospirillum brasilense

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the enzyme is more active in buffer containing 100 mM K+ in comparison to 100 mM Na+ Azospirillum brasilense
Mg2+ activates Azospirillum brasilense
Mn2+ activates, 5 mM used in assay conditions Azospirillum brasilense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
710000
-
 gel filtration Azospirillum brasilense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Azospirillum brasilense the enzyme probably operates in the direction of pyruvate and NADPH production under physiological conditions pyruvate + CO2 + NADPH + H+
-
 r
(S)-malate + NADP+ Azospirillum brasilense FP2 the enzyme probably operates in the direction of pyruvate and NADPH production under physiological conditions pyruvate + CO2 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-
Azospirillum brasilense FP2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin column chromatography Azospirillum brasilense
His-tagged enzyme is purified by HiTrap chelating Ni2+ column chromatography, untagged enzyme is partially purified by DEAE column chromatography Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
 Azospirillum brasilense pyruvate + CO2 + NADPH + H+
-
 r
(S)-malate + NADP+ the enzyme probably operates in the direction of pyruvate and NADPH production under physiological conditions Azospirillum brasilense pyruvate + CO2 + NADPH + H+
-
 r
(S)-malate + NADP+
-
 Azospirillum brasilense FP2 pyruvate + CO2 + NADPH + H+
-
 r
(S)-malate + NADP+ the enzyme probably operates in the direction of pyruvate and NADPH production under physiological conditions Azospirillum brasilense FP2 pyruvate + CO2 + NADPH + H+
-
 r
pyruvate + CO2 + NADPH + H+ the reverse reaction is very slow Azospirillum brasilense (S)-malate + NADP+
-
 r
pyruvate + CO2 + NADPH + H+ the reverse reaction is very slow Azospirillum brasilense FP2 (S)-malate + NADP+
-
 r

Subunits

Subunits Comment Organism
homooctamer 8 * 83000, SDS-PAGE Azospirillum brasilense

Synonyms

Synonyms Comment Organism
MaeB1
-
 Azospirillum brasilense
NADP-dependent malic enzyme
-
 Azospirillum brasilense
TME
-
 Azospirillum brasilense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 pyruvate at pH 8.0 and 30°C Azospirillum brasilense
327
-
 (S)-malate at pH 8.0 and 30°C Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
NADP+ the enzyme preferentially uses NADP+ as co-factor Azospirillum brasilense
NADPH
-
 Azospirillum brasilense

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.139
-
 acetyl-CoA at pH 8.0 and 30°C Azospirillum brasilense