Cloned (Comment) | Organism |
---|---|
genes NADP-ME1, NADP-ME2, and NADP-ME3, quantitative RT-PCR expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of knock-out mutants nadp-me2.1 and -2.2 by Agrobacterium tumefaciens strain GV3101-mediated transformation using the vacuum infiltration method | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | NADP-malic enzyme 2 | Arabidopsis thaliana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Arabidopsis thaliana | - |
pyruvate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isozymes NADP-ME1, NADP-ME2, and NADP-ME3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative NADP-malic enzyme activity in rosette leaves of Arabidopsis thaliana wild-type, loss-of-function (nadp-me2.1 and -2.2), complemented (nadp-me2.1 ? ME2-1 and -2) and overexpressing (ME2-1 and -2) lines, overview | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADP-malic enzyme | - |
Arabidopsis thaliana |
NADP-malic enzyme 2 | - |
Arabidopsis thaliana |
NADP-ME | - |
Arabidopsis thaliana |
NADP-ME1 | - |
Arabidopsis thaliana |
NADP-ME2 | - |
Arabidopsis thaliana |
NADP-ME3 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum, transient apoplastic reactive oxygen species production after elicitation and callose papilla formation after infection are dampened in mutant nadp-me2 | Arabidopsis thaliana |
additional information | analysis of transcriptional co-response patterns related NADP-ME2 to plant defence responses, overview | Arabidopsis thaliana |
physiological function | ADP-ME2 is an important player in plant basal defence, where it is involved in the generation of reactive oxygen species, NADP-ME2 is dispensable for later defence responses, overview | Arabidopsis thaliana |