Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.15 | - |
(3R,3S)-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
4.94 | - |
meso-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
12.06 | - |
(3R,3S)-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
14.49 | - |
glycerol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
37.39 | - |
meso-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
93.62 | - |
glycerol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
119.3 | - |
(2R,3R)-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
236.4 | - |
(2R,3R)-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | Klebsiella pneumoniae | - |
(3R)-acetoin + NADH + H+ | - |
? | |
(2R,3R)-butane-2,3-diol + NAD+ | Klebsiella pneumoniae ATCC 25955 | - |
(3R)-acetoin + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae ATCC 25955 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Sephadex G-25 gel filtration | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae | (3R)-acetoin + NADH + H+ | - |
? | |
(2R,3R)-butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae ATCC 25955 | (3R)-acetoin + NADH + H+ | - |
? | |
(3R,3S)-butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae | (3R,3S)-acetoin + NADH + H+ | - |
? | |
(3R,3S)-butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae ATCC 25955 | (3R,3S)-acetoin + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Klebsiella pneumoniae | ? | - |
? | |
glycerol + NAD+ | - |
Klebsiella pneumoniae ATCC 25955 | ? | - |
? | |
meso-butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae | (3S)-acetoin + NADH + H+ | - |
? | |
meso-butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae ATCC 25955 | (3S)-acetoin + NADH + H+ | - |
? | |
additional information | no activity with (2S,3S)-butane-2,3-diol or diacetyl | Klebsiella pneumoniae | ? | - |
? | |
additional information | no activity with (2S,3S)-butane-2,3-diol or diacetyl | Klebsiella pneumoniae ATCC 25955 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Klebsiella pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
DHAD | - |
Klebsiella pneumoniae |
GldA | - |
Klebsiella pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.68 | - |
(3R,3S)-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
3.82 | - |
(2R,3R)-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
4.67 | - |
meso-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
5.8 | - |
(2R,3R)-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
10.38 | - |
(3R,3S)-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
10.51 | - |
glycerol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
15.33 | - |
meso-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
18.88 | - |
glycerol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella pneumoniae |
Organism | Comment | Expression |
---|---|---|
Klebsiella pneumoniae | the enzyme gene dhaD is highly induced by glycerol | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
(2R,3R)-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
0.03 | - |
(2R,3R)-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
0.11 | - |
glycerol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
0.13 | - |
meso-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
0.31 | - |
(3R,3S)-butane-2,3-diol | enzyme DhaD, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
1.3 | - |
glycerol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
2.51 | - |
(3R,3S)-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae | |
3.11 | - |
meso-butane-2,3-diol | enzyme GldA, at pH 9.0 and 30°C | Klebsiella pneumoniae |