Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
NADH | - |
Pyrococcus furiosus | |
0.6 | - |
NAD+ | - |
Pyrococcus furiosus | |
6.5 | - |
acetoin | - |
Pyrococcus furiosus | |
86.8 | - |
2,3-Butanediol | - |
Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Pyrococcus furiosus |
32000 | - |
1 * 32000, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-butanediol + NAD+ | - |
Pyrococcus furiosus | acetoin + NADH + H+ | - |
r | |
2-pentanone + NADH + H+ | - |
Pyrococcus furiosus | (S)-2-pentanol + NAD+ | - |
? | |
acetoin + NAD+ | 18% of the activity with 2,3-butanediol, in the reverse reaction 150% of the activity with acetoin | Pyrococcus furiosus | diacetyl + NADH | - |
r | |
cyclohexanone + NADH | 38% of the activity with acetoin | Pyrococcus furiosus | ? | - |
? | |
dihydroxyacetone + NADH | 36% of the activity with acetoin | Pyrococcus furiosus | ? | - |
? | |
dihydroxyacetone phosphate + NADH | 82% of the activity with acetoin | Pyrococcus furiosus | ? | - |
? | |
pyruvic aldehyde + NADH | 81% of the activity with acetoin | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
catalytic activity increases up to | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life: 130 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
reduction of ketones | Pyrococcus furiosus |
8.8 | - |
oxidation of secondary alcohols | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can use NAD(H) and NADP(H) as cofactor, clear preference for NADH | Pyrococcus furiosus | |
NADH | can use NAD(H) and NADP(H) as cofactor, clear preference for NADH | Pyrococcus furiosus | |
NADP+ | 14% of the activity with NAD+ | Pyrococcus furiosus |